A Matter of Origin - Identification of SEMA3A, BGLAP, SPP1 and PHEX As Distinctive Molecular Features Between Bone Site-specific Human Osteoblasts on Transcription Level

Overview

Journal Front Bioeng Biotechnol

Date 2022 Oct 17

PMID 36246375

Authors

Weiping Zhang

Sibylle Rau

Konstantinos Kotzagiorgis

Rene Rothweiler

Susanne Nahles

Eric Gottwald

Bernd Rolauffs

Thorsten Steinberg

Katja Nelson

Brigitte Altmann

Affiliations

Soon will be listed here.

Abstract

In oral and maxillofacial bone reconstruction, autografts from the iliac crest represent the gold standard due to their superior clinical performance, compared to autografts derived from other extraoral regions. Thus, the aim of our study was to identify putative differences between osteoblasts derived from alveolar (hOB-A) and iliac crest (hOB-IC) bone of the same donor (nine donors) by means of their molecular properties in 2D and 3D culture. We thereby focused on the gene expression of biomarkers involved in osteogenic differentiation, matrix formation and osteoclast modulation. Furthermore, we examined the transcriptional response to Vit.D3 in hOB-A and hOB-IC. Our results revealed different modulation modes of the biomarker expression in osteoblasts, namely cell origin/bone entity-dependent, and culture configuration- and/or time-dependent modulations. SEMA3A, SPP1, BGLAP and PHEX demonstrated the strongest dependence on cell origin. With respect to Vit.D3-effects, BGLAP, SPP1 and ALPL displayed the highest Vit.D3-responsiveness. In this context we demonstrated that the transcriptional Vit.D3-response concerning SPP1 and ALPL in human osteoblasts depended on the cell origin. The results indicate a higher bone remodeling activity of iliac crest than alveolar osteoblasts and support the growing evidence that a high osteoclast activity at the host-/donor bone interface may support graft integration.

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References

Sotiropoulou P, Fountos G, Martini N, Koukou V, Michail C, Kandarakis I . Bone calcium/phosphorus ratio determination using dual energy X-ray method. Phys Med. 2015; 31(3):307-13. DOI: 10.1016/j.ejmp.2015.01.019. View

Huggett J . The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. Clin Chem. 2020; 66(8):1012-1029. DOI: 10.1093/clinchem/hvaa125. View

Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H . Osteoprotection by semaphorin 3A. Nature. 2012; 485(7396):69-74. DOI: 10.1038/nature11000. View

Fretwurst T, Wanner L, Nahles S, Raguse J, Stricker A, Metzger M . A prospective study of factors influencing morbidity after iliac crest harvesting for oral onlay grafting. J Craniomaxillofac Surg. 2015; 43(5):705-9. DOI: 10.1016/j.jcms.2015.03.023. View

Figueiredo L, Pace R, DArros C, Rethore G, Guicheux J, Le Visage C . Assessing glucose and oxygen diffusion in hydrogels for the rational design of 3D stem cell scaffolds in regenerative medicine. J Tissue Eng Regen Med. 2018; 12(5):1238-1246. DOI: 10.1002/term.2656. View

Nkenke E, Neukam F . Autogenous bone harvesting and grafting in advanced jaw resorption: morbidity, resorption and implant survival. Eur J Oral Implantol. 2014; 7 Suppl 2:S203-17. View

Rammelt S, Neumann M, Hanisch U, Reinstorf A, Pompe W, Zwipp H . Osteocalcin enhances bone remodeling around hydroxyapatite/collagen composites. J Biomed Mater Res A. 2005; 73(3):284-94. DOI: 10.1002/jbm.a.30263. View

Sutton A, Zhang X, Dowd D, Kharode Y, Komm B, MacDonald P . Semaphorin 3B is a 1,25-Dihydroxyvitamin D3-induced gene in osteoblasts that promotes osteoclastogenesis and induces osteopenia in mice. Mol Endocrinol. 2008; 22(6):1370-81. PMC: 2422826. DOI: 10.1210/me.2007-0363. View

Singh A, Gill G, Kaur H, Amhmed M, Jakhu H . Role of osteopontin in bone remodeling and orthodontic tooth movement: a review. Prog Orthod. 2018; 19(1):18. PMC: 6015792. DOI: 10.1186/s40510-018-0216-2. View

10.

Crockett J, Rogers M, Coxon F, Hocking L, Helfrich M . Bone remodelling at a glance. J Cell Sci. 2011; 124(Pt 7):991-8. DOI: 10.1242/jcs.063032. View

11.

Hirao M, Hashimoto J, Yamasaki N, Ando W, Tsuboi H, Myoui A . Oxygen tension is an important mediator of the transformation of osteoblasts to osteocytes. J Bone Miner Metab. 2007; 25(5):266-76. DOI: 10.1007/s00774-007-0765-9. View

12.

Altmann B, Steinberg T, Giselbrecht S, Gottwald E, Tomakidi P, Bachle-Haas M . Promotion of osteoblast differentiation in 3D biomaterial micro-chip arrays comprising fibronectin-coated poly(methyl methacrylate) polycarbonate. Biomaterials. 2011; 32(34):8947-56. DOI: 10.1016/j.biomaterials.2011.08.023. View

13.

Boukhechba F, Balaguer T, Michiels J, Ackermann K, Quincey D, Bouler J . Human primary osteocyte differentiation in a 3D culture system. J Bone Miner Res. 2009; 24(11):1927-35. DOI: 10.1359/jbmr.090517. View

14.

Everts V, de Vries T, Helfrich M . Osteoclast heterogeneity: lessons from osteopetrosis and inflammatory conditions. Biochim Biophys Acta. 2009; 1792(8):757-65. DOI: 10.1016/j.bbadis.2009.05.004. View

15.

Ikpegbu E, Basta L, Clements D, Fleming R, Vincent T, Buttle D . FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression. J Cell Physiol. 2017; 233(7):5334-5347. PMC: 5900964. DOI: 10.1002/jcp.26345. View

16.

Giselbrecht S, Gietzelt T, Gottwald E, Trautmann C, Truckenmuller R, Weibezahn K . 3D tissue culture substrates produced by microthermoforming of pre-processed polymer films. Biomed Microdevices. 2006; 8(3):191-9. DOI: 10.1007/s10544-006-8174-8. View

17.

Komori T . Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx2. Int J Mol Sci. 2019; 20(7). PMC: 6480215. DOI: 10.3390/ijms20071694. View

18.

Shen Q, Christakos S . The vitamin D receptor, Runx2, and the Notch signaling pathway cooperate in the transcriptional regulation of osteopontin. J Biol Chem. 2005; 280(49):40589-98. DOI: 10.1074/jbc.M504166200. View

19.

Glowacki J, Lian J . Impaired recruitment and differentiation of osteoclast progenitors by osteocalcin-deplete bone implants. Cell Differ. 1987; 21(4):247-54. DOI: 10.1016/0045-6039(87)90479-9. View

20.

Maruyama Z, Yoshida C, Furuichi T, Amizuka N, Ito M, Fukuyama R . Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency. Dev Dyn. 2007; 236(7):1876-90. DOI: 10.1002/dvdy.21187. View