Whole-Genome Relationships Among Francisella Bacteria of Diverse Origins Define New Species and Provide Specific Regions for Detection

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2016 Nov 25

PMID 27881415

Citations 30

Authors

Jean F Challacombe

Jeannine M Petersen

La Verne Gallegos-Graves

David Hodge

Segaran Pillai

Cheryl R Kuske

Affiliations

Soon will be listed here.

Abstract

Importance: DNA-based detection and sequencing methods have identified thousands of new bacteria in the human body and the environment. In most cases, there are no cultured isolates that correspond to these sequences. While DNA-based approaches are highly sensitive, accurately assigning species is difficult without known near relatives for comparison. This ambiguity poses challenges for clinical cases, disease epidemics, and environmental surveillance, for which response times must be short. Many new Francisella isolates have been identified globally. However, their species designations and potential for causing human disease remain ambiguous. Through detailed genome comparisons, we identified features that differentiate F. tularensis from clinical and environmental Francisella isolates and provide a knowledge base for future comparison of Francisella organisms identified in clinical samples or environmental surveys.

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