Reorganized Genomic Taxonomy of Enables Design of Robust Environmental PCR Assays for Detection of

Overview

Journal Microorganisms

Specialty Microbiology

Date 2021 Jan 14

PMID 33440900

Citations 18

Authors

Caroline Ohrman

Jason W Sahl

Andreas Sjodin

Ingrid Uneklint

Rebecca Ballard

Linda Karlsson

Ryelan F McDonough

David Sundell

Kathleen Soria

Stina Backman

Kitty Chase

Bjorn Brindefalk

Shanmuga Sozhamannan

Adriana Vallesi

Emil Hagglund

Jose Gustavo Ramirez-Paredes

Johanna Thelaus

Duncan Colquhoun

Kerstin Myrtennas

Dawn Birdsell

Anders Johansson

David M Wagner

Mats Forsman

Affiliations

Soon will be listed here.

Abstract

In recent years, an increasing diversity of species has been recognized within the family . Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of in environmental samples, such as water and air filters.

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