Loss of Electrical Anisotropy is an Unrecognized Feature of Dystrophic Muscle That May Serve As a Convenient Index of Disease Status

Overview

Journal Clin Neurophysiol

Publisher Elsevier

Specialties Neurology
Psychiatry

Date 2016 Nov 9

PMID 27825055

Citations 6

Authors

Seward B Rutkove

Jim S Wu

Craig Zaidman

Kush Kapur

Sung Yim

Amy Pasternak

Lavanya Madabusi

Heather Szelag

Tim Harrington

Jia Li

Adam Pacheck

Basil T Darras

Affiliations

Soon will be listed here.

Abstract

Objective: We sought to understand the alteration in the anisotropic, or direction dependent, character of muscle as measured by electrical impedance myography (EIM) in subjects with Duchenne muscular dystrophy (DMD) and its potential to serve as a biomarker of disease status.

Methods: Thirty-six boys with DMD and 27 healthy controls were measured with EIM, with electrical current applied both parallel and perpendicular to the major muscle fiber direction. In addition, muscle extracted from 10 mdx and 10 wild-type mice were measured analogously.

Results: Normalized reactance anisotropy, a direction-dependent measure of membrane charge storage capability, was significantly lower in the four muscles of DMD subjects as compared to controls (p<0.01). Normalized reactance anisotropy also decreased with increasing age in DMD subjects (r=-0.36, p=0.031), but not in healthy boys. Analogous changes were observed in mdx mouse gastrocnemius as compared to wild type (p=0.019).

Conclusion: These results support that loss of electrical anisotropy is a previously unrecognized feature of dystrophic muscle.

Significance: Anisotropic alterations may offer novel indices to assist in neuromuscular disease diagnosis and to serve as easy-to-obtain biomarkers in clinical therapeutic trials.

Citing Articles

Machine learning-enhanced electrical impedance myography to diagnose and track spinal muscular atrophy progression.

Sonbas Cobb B, Kolb S, Rutkove S Physiol Meas. 2024; 45(9).

PMID: 39197472 PMC: 11446569. DOI: 10.1088/1361-6579/ad74d5.

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents.

Mortreux M, Nagy J, Zhong H, Sung D, Concepcion H, Leitner M J Vis Exp. 2022; (184).

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Estimating myofiber cross-sectional area and connective tissue deposition with electrical impedance myography: A study in D2-mdx mice.

Pandeya S, Nagy J, Riveros D, Semple C, Taylor R, Mortreux M Muscle Nerve. 2021; 63(6):941-950.

PMID: 33759456 PMC: 8883327. DOI: 10.1002/mus.27240.

Electrical impedance myography: A critical review and outlook.

Sanchez B, Martinsen O, Freeborn T, Furse C Clin Neurophysiol. 2021; 132(2):338-344.

PMID: 33450556 PMC: 7880888. DOI: 10.1016/j.clinph.2020.11.014.

Validity of bioelectrical impedance to estimate fat-free mass in boys with Duchenne muscular dystrophy.

Grilo E, Cunha T, Costa A, Araujo B, Lopes M, Maciel B PLoS One. 2020; 15(11):e0241722.

PMID: 33216757 PMC: 7679022. DOI: 10.1371/journal.pone.0241722.

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