Preliminary Source Tracking of Male-Specific (F) RNA Coliphage on Lettuce As a Surrogate of Enteric Viruses Using Reverse Transcription-PCR

Overview

Journal Food Environ Virol

Publisher Springer

Specialties Environmental Health
Microbiology
Nutritional Sciences

Date 2016 Nov 6

PMID 27815782

Citations 1

Authors

Mojgan Yazdi

Masoud Yavarmanesh

Masumeh Bahreini

Mohebbat Mohebbi

Affiliations

Soon will be listed here.

Abstract

The aim of this research was to preliminary track fecal source male-specific FRNA coliphages including human and animals in lettuce. At first, two published virus extraction procedures of ultracentrifugation and PEG precipitation were compared using DAL assay for determining the recovery efficiency in lettuce spiked artificially with three concentrations (10, 10, 10 pfu/100 ml) of MS2 coliphage. The results showed that PEG precipitation had the highest recovery in which the recovery efficiency at the spiked level of 10 pfu/100 ml was 16.63 %. Aqueous phase obtained from the final step of PEG method was applied for enumeration of coliphage and viral RNA extraction in naturally contaminated lettuce samples (N = 30) collected from two sources (market and farm). The samples were then analyzed based on (I, II, III, and IV primer sets) using RT-PCR method. Coliphages were detected in 9 (60 %) and 12 (80 %) out of 15 market and farm samples, respectively, using DAL assay, whereas male-specific FRNA coliphages were detected using the RT-PCR method in 9 (60 %) and 13 (86.6 %) out of 15 samples of market and farm, respectively. Based on the results, only genotype I of male-specific FRNA coliphages was detected in lettuce samples and no sample tested was positive for other genotypes (II, III, and IV).

Citing Articles

Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and .

Vesga F, Venegas C, Florez Martinez V, Sanchez-Alfonso A, Trespalacios A Heliyon. 2024; 10(17):e36526.

PMID: 39263095 PMC: 11387255. DOI: 10.1016/j.heliyon.2024.e36526.

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