PEPCK-C Reexpression in the Liver Counters Neonatal Hypoglycemia in Pck1 Mice, Unmasking Role in Non-gluconeogenic Tissues

Overview

Journal J Physiol Biochem

Specialties Biochemistry
Physiology

Date 2016 Oct 28

PMID 27785616

Citations 9

Authors

Jana Semakova

Petra Hyrossova

Andres Mendez-Lucas

Ernest Cutz

Jordi Bermudez

Shawn Burgess

Soledad Alcantara

Jose C Perales

Affiliations

Soon will be listed here.

Abstract

Whole body cytosolic phosphoenolpyruvate carboxykinase knockout (PEPCK-C KO) mice die early after birth with profound hypoglycemia therefore masking the role of PEPCK-C in adult, non-gluconeogenic tissues where it is expressed. To investigate whether PEPCK-C deletion in the liver was critically responsible for the hypoglycemic phenotype, we reexpress this enzyme in the liver of PEPCK-C KO pups by early postnatal administration of PEPCK-C-expressing adenovirus. This maneuver was sufficient to partially rescue hypoglycemia and allow the pups to survive and identifies the liver as a critical organ, and hypoglycemia as the critical pathomechanism, leading to early postnatal death in the whole-body PEPCK-C knockout mice. Pathology assessment of survivors also suggest a possible role for PEPCK-C in lung maturation and muscle metabolism.

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