Morphological and Molecular Characterisation of Myxobolus Pronini N. Sp. (Myxozoa: Myxobolidae) from the Abdominal Cavity and Visceral Serous Membranes of the Gibel Carp Carassius Auratus Gibelio (Bloch) in Russia and China

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2016 Oct 27

PMID 27782848

Citations 11

Authors

Xin-Hua Liu

Marina-D Batueva

Yuan-Li Zhao

Jin-Yong Zhang

Qian-Qian Zhang

Tong-Tong Li

Ai-Hua Li

Affiliations

Soon will be listed here.

Abstract

Background: Myxozoa is a well-known economically and ecologically important group of metazoan parasites, phylogenetically related to Cnidaria. High diversity of myxosporeans has been recorded in Russia and China; however, most of the species were solely morphologically characterised. Here, we identified a new gibel carp-infecting Myxobolus species and morphologically and molecularly compared the Russian and Chinese isolates of this new myxosporean.

Results: Myxobolus pronini n. sp. was found free in the abdominal cavity of Carassius auratus gibelio (Bloch, 1782) in Lake Baikal watershed, Russia, and embedded in the visceral serous membranes of the same fish species in Lake Taibai, Hubei province, China. The morphometric data of the plasmodia and mature spores exhibited some differences between the Russian and Chinese isolates, but SSU rDNA sequences indicated that these two geographical isolates are conspecific. The mature spores from the two locations are obovate in frontal view, with wider anterior than posterior end and lemon-shaped in sutural view. Spores of the Russian isolate were 14.3-16.2 (mean 15.1 ± 0.2) μm long, 9.6-10.8 (10.1 ± 0.1) μm wide and 6.4-7.4 (6.7 ± 0.15) μm thick; those of the Chinese isolate were 13.8-15.6 (14.7 ± 0.24) μm long, 9.6-13.3 (9.6 ± 0.65) μm wide and 6.2-7.2 (6.6 ± 0.16) μm thick. The newly-generated rDNA sequences (including SSU rDNA, ITS and LSU rDNA) from the two isolates represented some variations within the intraspecific range. Homology search by BLAST showed that the newly obtained rDNA sequences do not match any sequences available on GenBank. Phylogenetic analysis based on the aligned partial SSU rDNA sequences indicated that this novel species clustered with several gibel carp-infecting Myxobolus spp. with round anterior end of spores. Additionally, phylogenetic analysis based on all obtained ITS sequences showed that distinct genetic geographical differentiation occurred for this new parasite.

Conclusions: Myxobolus pronini n. sp. is described by integrating morphological, ecological and molecular evidence. Two geographical isolates of this species showed some morphological and genetic differences but within the intraspecific range of variation.

Citing Articles

Clinical, histopathological and phylogenetic analysis of Myxobolus lentisturalis (Myxozoa: Myxobolidae) infecting the musculature of farmed population of goldfish (Carassius auratus) in Iran: 2021-2022.

Rahmati-Holasoo H, Marandi A, Ebrahimzadeh Mousavi H, Arabkhazaeli F, Shokrpoor S, Ziafati Kafi Z BMC Vet Res. 2024; 20(1):361.

PMID: 39129031 PMC: 11318346. DOI: 10.1186/s12917-024-04218-1.

Diversity of myxozoans (Cnidaria) infecting Neotropical fishes in southern Mexico.

Alama-Bermejo G, Hernandez-Orts J, Garcia-Varela M, Oceguera-Figueroa A, Peckova H, Fiala I Sci Rep. 2023; 13(1):12106.

PMID: 37495605 PMC: 10372099. DOI: 10.1038/s41598-023-38482-2.

Myxozoan survey of thicklip grey mullet Chelon labrosus reinforces successful radiation of Myxobolus in mugiliform hosts.

Guimaraes J, Casal G, Alves A, Araujo C, Rocha S Parasite. 2023; 30:26.

PMID: 37401858 PMC: 10318956. DOI: 10.1051/parasite/2023029.

Morphological and Molecular Characterization of Myxobolus nkondjockei sp. nov. (Myxozoa: Myxobolidae), A Parasite of Labeo batesii Boulenger, 1911 (Teleostei: Cyprinidae) from Makombè River in Cameroon.

Lekeufack-Folefack G, Feudjio-Dongmo B, Tene-Fossog B, Fomena A, Wondji C, Al-Tamimi J Acta Parasitol. 2022; 67(4):1573-1583.

PMID: 36018469 DOI: 10.1007/s11686-022-00609-2.

Morphological and molecular characterization of a new species Myxobolus gutturocola n. sp. (Myxozoa: Myxobolidae) from the throat of Hypophthalmichthys molitrix in China.

Liu J, Zhang J, Zhao Y Parasitol Res. 2019; 118(3):773-781.

PMID: 30721355 DOI: 10.1007/s00436-019-06226-9.

References

Whipps C, Adlard R, Bryant M, Lester R, Findlay V, Kent M . First report of three Kudoa species from eastern Australia: Kudoa thyrsites from mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from sweeper (Pempheris ypsilychnus). J Eukaryot Microbiol. 2003; 50(3):215-9. DOI: 10.1111/j.1550-7408.2003.tb00120.x. View

Zhang J, Wang J, Li A, Gong X . Infection of Myxobolus turpisrotundus sp. n. in allogynogenetic gibel carp, Carassius auratus gibelio (Bloch), with revision of Myxobolus rotundus (s. l.) Nemeczek reported from C. auratus auratus (L.). J Fish Dis. 2010; 33(8):625-38. DOI: 10.1111/j.1365-2761.2010.01161.x. View

Zhao Y, Li N, Tang F, Dong J . Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa: Myxosporea) based on SSU rDNA sequences. Parasitol Res. 2013; 112(11):3817-23. PMC: 3825597. DOI: 10.1007/s00436-013-3569-y. View

Eiras J, Molnar K, Lu Y . Synopsis of the species of Myxobolus Butschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol. 2005; 61(1):1-46. DOI: 10.1007/s11230-004-6343-9. View

Bartosova P, Fiala I, Hypsa V . Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics. Mol Phylogenet Evol. 2009; 53(1):81-93. DOI: 10.1016/j.ympev.2009.05.018. View

Liu X, Zhang J, Batueva M, Voronin V . Supplemental description and molecular characterization of Myxobolus miyarii Kudo, 1919 (Myxosporea: Myxobolidae) infecting intestine of Amur catfish (Silurus asotus). Parasitol Res. 2015; 115(4):1547-56. DOI: 10.1007/s00436-015-4889-x. View

Urawa S, Freeman M, Johnson S, Jones S, Yokoyama H . Geographical variation in spore morphology, gene sequences, and host specificity of Myxobolus arcticus (Myxozoa) infecting salmonid nerve tissues. Dis Aquat Organ. 2011; 96(3):229-37. DOI: 10.3354/dao02398. View

Ronquist F, Huelsenbeck J . MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003; 19(12):1572-4. DOI: 10.1093/bioinformatics/btg180. View

Van der Auwera G, Chapelle S, De Wachter R . Structure of the large ribosomal subunit RNA of Phytophthora megasperma, and phylogeny of the oomycetes. FEBS Lett. 1994; 338(2):133-6. DOI: 10.1016/0014-5793(94)80350-1. View

10.

Posada D . jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008; 25(7):1253-6. DOI: 10.1093/molbev/msn083. View

11.

Bartosova-Sojkova P, Hrabcova M, Peckova H, Patra S, Kodadkova A, Jurajda P . Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics. Int J Parasitol. 2014; 44(8):565-77. DOI: 10.1016/j.ijpara.2014.04.005. View

12.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

13.

Shin S, Shirakashi S, Hamano S, Kato K, Lasso L, Yokoyama H . Phylogenetic study of the genus Kudoa (Myxozoa: Multivalvulida) with a description of Kudoa rayformis sp. nov. from the trunk muscle of Pacific sierra Scomberomorus sierra. Mol Phylogenet Evol. 2016; 98:337-45. DOI: 10.1016/j.ympev.2016.02.019. View

14.

Liu Y, Whipps C, Gu Z, Zeng L . Myxobolus turpisrotundus (Myxosporea: Bivalvulida) spores with caudal appendages: investigating the validity of the genus Henneguya with morphological and molecular evidence. Parasitol Res. 2010; 107(3):699-706. DOI: 10.1007/s00436-010-1924-9. View

15.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

16.

Eiras J, Zhang J, Molnar K . Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea, Myxobolidae) described between 2005 and 2013. Syst Parasitol. 2014; 88(1):11-36. DOI: 10.1007/s11230-014-9484-5. View

17.

Kent M, Andree K, Bartholomew J, El-Matbouli M, Desser S, Devlin R . Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol. 2001; 48(4):395-413. DOI: 10.1111/j.1550-7408.2001.tb00173.x. View

18.

Dykova I, Fiala I, Nie P . Myxobolus lentisuturalis sp. n. (Myxozoa: Myxobolidae), a new muscle-infecting species from the Prussian carp, Carassius gibelio from China. Folia Parasitol (Praha). 2003; 49(4):253-8. DOI: 10.14411/fp.2002.048. View

19.

Eszterbauer E . Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Organ. 2004; 58(1):35-40. DOI: 10.3354/dao058035. View

20.

Henderson M, Okamura B . The phylogeography of salmonid proliferative kidney disease in Europe and North America. Proc Biol Sci. 2004; 271(1549):1729-36. PMC: 1691782. DOI: 10.1098/rspb.2004.2677. View