Utilization of Iron (III)-doped Nanoshells for In vivo Marking of Nonpalpable Tumors Using a VX2 Rabbit Model

Overview

Journal Am J Surg

Specialty General Surgery

Date 2016 Oct 26

PMID 27776757

Citations 1

Authors

Erin P Ward

James Wang

Natalie Mendez

Jian Yang

Chris Barback

Jessica Wang-Rodriguez

William Trogler

Andrew C Kummel

Sarah Blair

Affiliations

Soon will be listed here.

Abstract

Background: We aimed to evaluate the potential for ultrasound (US) visible biodegradable nanoshells (NS) as an alternative to wire-guided localization for nonpalpable tumors in vivo.

Methods: VX2 tumor was injected in bilateral thighs of 22 New Zealand rabbits and after 5 to 10 days, 1 tumor was marked with a wire as a control and the contralateral tumor was injected with 1 mL of 500 nm gas-filled silica NS under Doppler US. Tumors were excised after 24 hours. Chi-square was used for significance, P = .05.

Results: One rabbit was excluded on postoperative day 1 due to equipment failure, no ill effects were observed from the NS. The NS were used to localize and resect 100% of marked tissue, 4/21 wires were displaced (P < .05).

Conclusions: We have shown that preoperatively injected US visible silica NS can be successfully used to mark nonpalpable tumors in vivo more consistently than WL.

Citing Articles

Doppler Ultrasound-Visible SignalMark Microspheres are Better Identified than HydroMARK Clips in a Simulated Intraoperative Setting in Breast and Lung Tissue.

Voss R, Ward E, Ojeda-Fournier H, Blair S Ann Surg Oncol. 2018; 25(12):3740-3746.

PMID: 30178390 PMC: 6277056. DOI: 10.1245/s10434-018-6707-z.

References

Liberman A, Martinez H, Ta C, Barback C, Mattrey R, Kono Y . Hollow silica and silica-boron nano/microparticles for contrast-enhanced ultrasound to detect small tumors. Biomaterials. 2012; 33(20):5124-9. PMC: 3588157. DOI: 10.1016/j.biomaterials.2012.03.066. View

Myers E, Moorman P, Gierisch J, Havrilesky L, Grimm L, Ghate S . Benefits and Harms of Breast Cancer Screening: A Systematic Review. JAMA. 2015; 314(15):1615-34. DOI: 10.1001/jama.2015.13183. View

Pan H, Wu N, Ding H, Ding Q, Dai J, Ling L . Intraoperative ultrasound guidance is associated with clear lumpectomy margins for breast cancer: a systematic review and meta-analysis. PLoS One. 2013; 8(9):e74028. PMC: 3779206. DOI: 10.1371/journal.pone.0074028. View

Fisher B, Anderson S, Bryant J, Margolese R, Deutsch M, Fisher E . Twenty-year follow-up of a randomized trial comparing total mastectomy, lumpectomy, and lumpectomy plus irradiation for the treatment of invasive breast cancer. N Engl J Med. 2002; 347(16):1233-41. DOI: 10.1056/NEJMoa022152. View

DeSantis C, Ma J, Bryan L, Jemal A . Breast cancer statistics, 2013. CA Cancer J Clin. 2013; 64(1):52-62. DOI: 10.3322/caac.21203. View

Mullenix P, Cuadrado D, Steele S, Martin M, See C, Beitler A . Secondary operations are frequently required to complete the surgical phase of therapy in the era of breast conservation and sentinel lymph node biopsy. Am J Surg. 2004; 187(5):643-6. DOI: 10.1016/j.amjsurg.2004.01.003. View

Liberman L, Kaplan J, Van Zee K, Morris E, LaTrenta L, Abramson A . Bracketing wires for preoperative breast needle localization. AJR Am J Roentgenol. 2001; 177(3):565-72. DOI: 10.2214/ajr.177.3.1770565. View

Volders J, Haloua M, Krekel N, Meijer S, van den Tol P . Current status of ultrasound-guided surgery in the treatment of breast cancer. World J Clin Oncol. 2016; 7(1):44-53. PMC: 4734937. DOI: 10.5306/wjco.v7.i1.44. View

Chan B, Wiseberg-Firtell J, Jois R, Jensen K, Audisio R . Localization techniques for guided surgical excision of non-palpable breast lesions. Cochrane Database Syst Rev. 2016; (12):CD009206. PMC: 8881987. DOI: 10.1002/14651858.CD009206.pub2. View

10.

Liberman A, Wu Z, Barback C, Viveros R, Blair S, Ellies L . Color Doppler ultrasound and gamma imaging of intratumorally injected 500 nm iron-silica nanoshells. ACS Nano. 2013; 7(7):6367-77. PMC: 3777724. DOI: 10.1021/nn402507d. View

11.

Sharek D, Zuley M, Zhang J, Soran A, Ahrendt G, Ganott M . Radioactive seed localization versus wire localization for lumpectomies: a comparison of outcomes. AJR Am J Roentgenol. 2015; 204(4):872-7. DOI: 10.2214/AJR.14.12743. View

12.

Liberman A, Wang J, Lu N, Viveros R, Allen C, Mattrey R . Mechanically Tunable Hollow Silica Ultrathin Nanoshells for Ultrasound Contrast Agents. Adv Funct Mater. 2016; 25(26):4049-4057. PMC: 4778971. DOI: 10.1002/adfm.201500610. View

13.

Eggemann H, Costa S, Ignatov A . Ultrasound-Guided Versus Wire-Guided Breast-Conserving Surgery for Nonpalpable Breast Cancer. Clin Breast Cancer. 2015; 16(1):e1-6. DOI: 10.1016/j.clbc.2015.09.001. View

14.

Martinez H, Kono Y, Blair S, Sandoval S, Wang-Rodriguez J, Mattrey R . Hard shell gas-filled contrast enhancement particles for colour Doppler ultrasound imaging of tumors. Medchemcomm. 2011; 1(4):266-270. PMC: 3155153. DOI: 10.1039/c0md00139b. View

15.

Yang S, Yang K, Li Y, Zhang Y, He X, Song A . Breast conservation therapy for stage I or stage II breast cancer: a meta-analysis of randomized controlled trials. Ann Oncol. 2008; 19(6):1039-44. DOI: 10.1093/annonc/mdm573. View

16.

Ananthakrishnan P, Balci F, Crowe J . Optimizing surgical margins in breast conservation. Int J Surg Oncol. 2013; 2012:585670. PMC: 3523540. DOI: 10.1155/2012/585670. View

17.

Yang J, Sandoval S, Alfaro J, Aschemeyer S, Liberman A, Martin D . Red-luminescent europium (III) doped silica nanoshells: synthesis, characterization, and their interaction with HeLa cells. J Biomed Opt. 2011; 16(6):066012. PMC: 3133801. DOI: 10.1117/1.3593003. View

18.

Lovrics P, Goldsmith C, Hodgson N, McCready D, Gohla G, Boylan C . A multicentered, randomized, controlled trial comparing radioguided seed localization to standard wire localization for nonpalpable, invasive and in situ breast carcinomas. Ann Surg Oncol. 2011; 18(12):3407-14. DOI: 10.1245/s10434-011-1699-y. View

19.

Gangnon R, Sprague B, Stout N, Alagoz O, Weedon-Fekjaer H, Holford T . The contribution of mammography screening to breast cancer incidence trends in the United States: an updated age-period-cohort model. Cancer Epidemiol Biomarkers Prev. 2015; 24(6):905-12. PMC: 4489135. DOI: 10.1158/1055-9965.EPI-14-1286. View

20.

Lovrics P, Cornacchi S, Vora R, Goldsmith C, Kahnamoui K . Systematic review of radioguided surgery for non-palpable breast cancer. Eur J Surg Oncol. 2011; 37(5):388-97. DOI: 10.1016/j.ejso.2011.01.018. View