Neurobiochemical Changes in the Vicinity of a Nanostructured Neural Implant

Overview

Journal Sci Rep

Specialty Science

Date 2016 Oct 25

PMID 27775024

Citations 14

Authors

Zsofia Berces

Kinga Toth

Gergely Marton

Ildiko Pal

Balint Kovats-Megyesi

Zoltan Fekete

Istvan Ulbert

Anita Pongracz

Affiliations

Soon will be listed here.

Abstract

Neural interface technologies including recording and stimulation electrodes are currently in the early phase of clinical trials aiming to help patients with spinal cord injuries, degenerative disorders, strokes interrupting descending motor pathways, or limb amputations. Their lifetime is of key importance; however, it is limited by the foreign body response of the tissue causing the loss of neurons and a reactive astrogliosis around the implant surface. Improving the biocompatibility of implant surfaces, especially promoting neuronal attachment and regeneration is therefore essential. In our work, bioactive properties of implanted black polySi nanostructured surfaces (520-800 nm long nanopillars with a diameter of 150-200 nm) were investigated and compared to microstructured Si surfaces in eight-week-long in vivo experiments. Glial encapsulation and local neuronal cell loss were characterised using GFAP and NeuN immunostaining respectively, followed by systematic image analysis. Regarding the severity of gliosis, no significant difference was observed in the vicinity of the different implant surfaces, however, the number of surviving neurons close to the nanostructured surface was higher than that of the microstructured ones. Our results imply that the functionality of implanted microelectrodes covered by Si nanopillars may lead to improved long-term recordings.

Citing Articles

Biomaterials for neuroengineering: applications and challenges.

Wu H, Feng E, Yin H, Zhang Y, Chen G, Zhu B Regen Biomater. 2025; 12:rbae137.

PMID: 40007617 PMC: 11855295. DOI: 10.1093/rb/rbae137.

biocompatibility evaluation of functional electrically stimulating microelectrodes on primary glia.

Tsui C, Mirkiani S, Roszko D, Churchward M, Mushahwar V, Todd K Front Bioeng Biotechnol. 2024; 12:1351087.

PMID: 38314352 PMC: 10834782. DOI: 10.3389/fbioe.2024.1351087.

Manufacturing Processes of Implantable Microelectrode Array for In Vivo Neural Electrophysiological Recordings and Stimulation: A State-Of-the-Art Review.

Yi D, Yao Y, Wang Y, Chen L J Micro Nanomanuf. 2023; 10(4):041001.

PMID: 37860671 PMC: 10583290. DOI: 10.1115/1.4063179.

Immunohistological responses in mice implanted with Parylene HT - ITO ECoG devices.

Madarasz M, Fedor F, Fekete Z, Rozsa B Front Neurosci. 2023; 17:1209913.

PMID: 37746144 PMC: 10513038. DOI: 10.3389/fnins.2023.1209913.

Commonly Overlooked Factors in Biocompatibility Studies of Neural Implants.

Kumosa L Adv Sci (Weinh). 2023; 10(6):e2205095.

PMID: 36596702 PMC: 9951391. DOI: 10.1002/advs.202205095.

References

Maxwell W, Follows R, Ashhurst D, Berry M . The response of the cerebral hemisphere of the rat to injury. I. The mature rat. Philos Trans R Soc Lond B Biol Sci. 1990; 328(1250):479-500. DOI: 10.1098/rstb.1990.0121. View

Jacque C, Vinner C, Kujas M, Raoul M, RACADOT J, Baumann N . Determination of glial fibrillary acidic protein (GFAP) in human brain tumors. J Neurol Sci. 1978; 35(1):147-55. DOI: 10.1016/0022-510x(78)90107-7. View

Ma J, Cui F, Liu B, Xu Q . Atomic force and confocal microscopy for the study of cortical cells cultured on silicon wafers. J Mater Sci Mater Med. 2007; 18(5):851-6. DOI: 10.1007/s10856-006-0071-4. View

Lin C, Lin J, Chen Y, Shen H, Wei L, Yeh Y . Hyaluronic acid inhibits the glial scar formation after brain damage with tissue loss in rats. Surg Neurol. 2009; 72 Suppl 2:S50-4. DOI: 10.1016/j.wneu.2009.09.004. View

Potter K, Buck A, Self W, Capadona J . Stab injury and device implantation within the brain results in inversely multiphasic neuroinflammatory and neurodegenerative responses. J Neural Eng. 2012; 9(4):046020. DOI: 10.1088/1741-2560/9/4/046020. View

Berthing T, Bonde S, Sorensen C, Utko P, Nygard J, Martinez K . Intact mammalian cell function on semiconductor nanowire arrays: new perspectives for cell-based biosensing. Small. 2011; 7(5):640-7. DOI: 10.1002/smll.201001642. View

Li W, Tang Q, Jadhav A, Narang A, Qian W, Shi P . Large-scale topographical screen for investigation of physical neural-guidance cues. Sci Rep. 2015; 5:8644. PMC: 4345323. DOI: 10.1038/srep08644. View

Edell D, Toi V, McNeil V, CLARK L . Factors influencing the biocompatibility of insertable silicon microshafts in cerebral cortex. IEEE Trans Biomed Eng. 1992; 39(6):635-43. DOI: 10.1109/10.141202. View

Donoghue J . Bridging the brain to the world: a perspective on neural interface systems. Neuron. 2008; 60(3):511-21. DOI: 10.1016/j.neuron.2008.10.037. View

10.

Moxon K, Hallman S, Aslani A, Kalkhoran N, Lelkes P . Bioactive properties of nanostructured porous silicon for enhancing electrode to neuron interfaces. J Biomater Sci Polym Ed. 2007; 18(10):1263-81. DOI: 10.1163/156856207782177882. View

11.

Almquist B, Melosh N . Fusion of biomimetic stealth probes into lipid bilayer cores. Proc Natl Acad Sci U S A. 2010; 107(13):5815-20. PMC: 2851869. DOI: 10.1073/pnas.0909250107. View

12.

Dowell-Mesfin N, Abdul-Karim M, Turner A, Schanz S, Craighead H, Roysam B . Topographically modified surfaces affect orientation and growth of hippocampal neurons. J Neural Eng. 2005; 1(2):78-90. DOI: 10.1088/1741-2560/1/2/003. View

13.

Kim D, Provenzano P, Smith C, Levchenko A . Matrix nanotopography as a regulator of cell function. J Cell Biol. 2012; 197(3):351-60. PMC: 3341161. DOI: 10.1083/jcb.201108062. View

14.

Moxon K, Kalkhoran N, Markert M, Sambito M, McKenzie J, Webster J . Nanostructured surface modification of ceramic-based microelectrodes to enhance biocompatibility for a direct brain-machine interface. IEEE Trans Biomed Eng. 2004; 51(6):881-9. DOI: 10.1109/TBME.2004.827465. View

15.

McConnell G, Rees H, Levey A, Gutekunst C, Gross R, Bellamkonda R . Implanted neural electrodes cause chronic, local inflammation that is correlated with local neurodegeneration. J Neural Eng. 2009; 6(5):056003. DOI: 10.1088/1741-2560/6/5/056003. View

16.

Donoghue J . Connecting cortex to machines: recent advances in brain interfaces. Nat Neurosci. 2002; 5 Suppl:1085-8. DOI: 10.1038/nn947. View

17.

Ludwig K, Uram J, Yang J, Martin D, Kipke D . Chronic neural recordings using silicon microelectrode arrays electrochemically deposited with a poly(3,4-ethylenedioxythiophene) (PEDOT) film. J Neural Eng. 2006; 3(1):59-70. DOI: 10.1088/1741-2560/3/1/007. View

18.

Winslow B, Tresco P . Quantitative analysis of the tissue response to chronically implanted microwire electrodes in rat cortex. Biomaterials. 2009; 31(7):1558-67. DOI: 10.1016/j.biomaterials.2009.11.049. View

19.

Sofroniew M . Molecular dissection of reactive astrogliosis and glial scar formation. Trends Neurosci. 2009; 32(12):638-47. PMC: 2787735. DOI: 10.1016/j.tins.2009.08.002. View

20.

Burda J, Sofroniew M . Reactive gliosis and the multicellular response to CNS damage and disease. Neuron. 2014; 81(2):229-48. PMC: 3984950. DOI: 10.1016/j.neuron.2013.12.034. View