Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of MiRNAs in Gastric Cancer Cell Line

Overview

Journal Iran J Cancer Prev

Specialty Oncology

Date 2016 Oct 21

PMID 27761206

Citations 22

Authors

Farideh Mohammadian

Alireza Abhari

Hassan Dariushnejad

Alireza Nikanfar

Younes Pilehvar-Soltanahmadi

Nosratollah Zarghami

Affiliations

Soon will be listed here.

Abstract

Background: Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line.

Objectives: The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line.

Methods: Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR. The assessment of cytotoxicity on the growth of the human gastric cell line was carried out through MTT assay. After treating the cells with a prearranged amount of pure and encapsulated chrysin, RNA was extracted and the expressions of miR-22, miR-34a and miR-126 were measured by using real-time PCR.

Results: With regard to the amount of the chrysin loaded in PLGA-PEG nanoparticles, IC50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. This finding has been proved through the further increase of miR-22, miR-34a and miR-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin.

Conclusions: In this study, we revealed that the PLGA-PEG-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line.

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