A Rare Subset of Skin-tropic Regulatory T Cells Expressing Il10/Gzmb Inhibits the Cutaneous Immune Response

Overview

Journal Sci Rep

Specialty Science

Date 2016 Oct 21

PMID 27756896

Citations 17

Authors

Ryoyo Ikebuchi

Shunsuke Teraguchi

Alexis Vandenbon

Tetsuya Honda

Francis H W Shand

Yasutaka Nakanishi

Takeshi Watanabe

Michio Tomura

Affiliations

Soon will be listed here.

Abstract

Foxp3 regulatory T cells (Tregs) migrating from the skin to the draining lymph node (dLN) have a strong immunosuppressive effect on the cutaneous immune response. However, the subpopulations responsible for their inhibitory function remain unclear. We investigated single-cell gene expression heterogeneity in Tregs from the dLN of inflamed skin in a contact hypersensitivity model. The immunosuppressive genes Ctla4 and Tgfb1 were expressed in the majority of Tregs. Although Il10-expressing Tregs were rare, unexpectedly, the majority of Il10-expressing Tregs co-expressed Gzmb and displayed Th1-skewing. Single-cell profiling revealed that CD43 CCR5 Tregs represented the main subset within the Il10/Gzmb-expressing cell population in the dLN. Moreover, CD43 CCR5 CXCR3 Tregs expressed skin-tropic chemokine receptors, were preferentially retained in inflamed skin and downregulated the cutaneous immune response. The identification of a rare Treg subset co-expressing multiple immunosuppressive molecules and having tissue-remaining capacity offers a novel strategy for the control of skin inflammatory responses.

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