The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract Through Upregulating KLF6 Gene Expression

Overview

Journal Nutrients

Date 2016 Oct 1

PMID 27690088

Citations 11

Authors

Yue Wang

Xia-Nan Zhang

Wen-Hua Xie

Yi-Xiong Zheng

Jin-Ping Cao

Pei-Rang Cao

Qing-Jun Chen

Xian Li

Chong-De Sun

Affiliations

Soon will be listed here.

Abstract

To investigate the antitumor effect of anthocyanins extracted from Chinese bayberry fruit ( Sieb. et Zucc.), a nude mouse tumor xenograft model was established. Treatments with C3G (cyanidin-3-glucoside, an anthocyanin) significantly suppressed the growth of SGC-7901 tumor xenografts in a dose-dependent manner. Immunohistochemical staining showed a significant increase in p21 expression, indicating that the cell cycle of tumor xenografts was inhibited. qPCR screening showed that C3G treatment up-regulated the expression of the gene, which is an important tumor suppressor gene inactivated in many human cancers. Western blot showed that C3G treatments markedly increased KLF6 and p21 protein levels, inhibited CDK4 and Cyclin D1 expression, but did not notably change the expression of p53. These results indicated that KLF6 up-regulates p21 in a p53-independent manner and significantly reduces tumor proliferation. This study provides important information for the possible mechanism of C3G-induced antitumor activity against gastric adenocarcinoma in vivo.

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