Induction of Varicella Zoster Virus DNA Replication in Dissociated Human Trigeminal Ganglia

Overview

Journal J Neurovirol

Publisher Springer

Specialties Microbiology
Neurology

Date 2016 Sep 30

PMID 27683235

Citations 6

Authors

Randall J Cohrs

Hussain Badani

Nicholas L Baird

Teresa M White

Bridget Sanford

Don Gilden

Affiliations

Soon will be listed here.

Abstract

Varicella zoster virus (VZV), a human neurotropic alphaherpesvirus, becomes latent after primary infection and reactivates to produce zoster. To study VZV latency and reactivation, human trigeminal ganglia removed within 24 h after death were mechanically dissociated, randomly distributed into six-well tissue culture plates and incubated with reagents to inactivate nerve growth factor (NGF) or phosphoinositide 3-kinase (PI3-kinase) pathways. At 5 days, VZV DNA increased in control and PI3-kinase inhibitor-treated cultures to the same extent, but was significantly more abundant in anti-NGF-treated cultures (p = 0.001). Overall, VZV DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent.

Citing Articles

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