Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry

Overview

Journal Lipids

Specialty Biochemistry

Date 2016 Sep 28

PMID 27671161

Citations 9

Authors

Yiu Yiu Lee

Jean-Marie Galano

Camille Oger

Claire Vigor

Reversat Guillaume

Jerome Roy

Jean-Yves Le Guennec

Thierry Durand

Jetty Chung-Yung Lee

Affiliations

Soon will be listed here.

Abstract

Oxygenated lipid mediators released from non-enzymatic peroxidation of polyunsaturated fatty acids (PUFA) are known to have functional roles in humans. Notably, among these lipid mediators, isoprostanes molecules are robust biomarkers of oxidative stress but those from n-3 PUFA are also bioactive molecules. In order to identify and assess the isoprostanes, the use of mass spectrometry (MS) for analysis is preferable and has been used for over two decades. Gas chromatography (GC) is commonly coupled to the MS to separate the derivatized isoprostanes of interest in biological samples. In order to increase the accuracy of the analytical performance, GC-MS/MS was also applied. Lately, MS or MS/MS has been coupled with high-performance liquid chromatography to assess multiple isoprostane molecules in a single biological sample without derivatization process. However, there are limitations for the use of LC-MS/MS in the measurement of plasma isoprostanes, which will be discussed in this review.

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