Engineering New Balancer Chromosomes in C. Elegans Via CRISPR/Cas9

Overview

Journal Sci Rep

Specialty Science

Date 2016 Sep 22

PMID 27650892

Citations 18

Authors

Satoru Iwata

Sawako Yoshina

Yuji Suehiro

Sayaka Hori

Shohei Mitani

Affiliations

Soon will be listed here.

Abstract

Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes.

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