Targeted Chromosomal Rearrangements Via Combinatorial Use of CRISPR/Cas9 and Cre/ Technologies in

Overview

Journal G3 (Bethesda)

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2018 Jun 29

PMID 29950430

Citations 6

Authors

Xiangyang Chen

Shimiao Liao

Xinya Huang

Ting Xu

Xuezhu Feng

Shouhong Guang

Affiliations

Soon will be listed here.

Abstract

Rearranged chromosomes have been applied to construct genetic balancers to manipulate essential genes in Although much effort has been put into constructing balancer chromosomes, approximately 6% (map units) of the genome has not been covered, and this area lies mostly in pairing centers (PCs). Here, we developed a method for conditional chromosomal engineering through combinatorial use of the CRISPR/Cas9 and Cre/ technologies. Functional DNA fragments containing sequences were inserted into designated genomic loci using a modified counterselection (cs)-CRISPR method. Then, heat-shock-induced Cre recombinase induced an inversion of the chromosomal region between the two sites. The chromosomal inversions were subsequently detected by the appearance of pharyngeal GFP. Through this method, we have successfully generated several chromosomal inversion lines, providing valuable resources for studying essential genes in pairing centers.

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