Microglial and Neural Differentiation in Human Teratomas

Overview

Journal Acta Neuropathol

Specialty Neurology

Date 1989 Jan 1

PMID 2763798

Citations 2

Authors

T Sangruchi

R A Sobel

Affiliations

Soon will be listed here.

Abstract

Ten human teratomas arising outside the central nervous system (CNS) were studied using a panel of immunohistochemical, and lectin histochemical stains to determine the relationship of the presence of microglia to markers of neural maturity or differentiation. Microglia, identified by silver carbonate, Ricinus communis agglutinin-1 (RCA-1), or both were found in eight out of ten cases. They were common in mature areas which also had S-100, glial fibrillary acidic protein, vimentin, neurofilament, and synaptophysin immunostaining. Microglia were distinguished from macrophages in necrotic foci. Cells which were RCA-1 positive and silver carbonate positive were found in immature neural tissues but these lacked all typical features of microglia. These observations indicate that true microglia are frequent in nonCNS teratomas and that they are found in association with other indicators of neural maturation. The presence of possible precursors in immature areas suggests that microglia undergo maturation concurrent with neural differentiation in these tumors.

Citing Articles

Immunophenotypic features of immaturity of neural elements in ovarian teratoma.

Takayama Y, Matsumura N, Nobusawa S, Ikota H, Minegishi T, Yokoo H Virchows Arch. 2015; 468(3):337-43.

PMID: 26638157 DOI: 10.1007/s00428-015-1891-8.

Selective apoptosis of pluripotent mouse and human stem cells by novel ceramide analogues prevents teratoma formation and enriches for neural precursors in ES cell-derived neural transplants.

Bieberich E, Silva J, Wang G, Krishnamurthy K, Condie B J Cell Biol. 2004; 167(4):723-34.

PMID: 15545317 PMC: 2172580. DOI: 10.1083/jcb.200405144.

References

Sternberger N, Itoyama Y, KIES M, WEBSTER H . Myelin basic protein demonstrated immunocytochemically in oligodendroglia prior to myelin sheath formation. Proc Natl Acad Sci U S A. 1978; 75(5):2521-4. PMC: 392586. DOI: 10.1073/pnas.75.5.2521. View

Schiffer D, Giordana M, Mauro A, Migheli A, Germano I, Giaccone G . Immunohistochemical demonstration of vimentin in human cerebral tumors. Acta Neuropathol. 1986; 70(3-4):209-19. DOI: 10.1007/BF00686074. View

Sobel R, Blanchette B, Bhan A, Colvin R . The immunopathology of experimental allergic encephalomyelitis. I. Quantitative analysis of inflammatory cells in situ. J Immunol. 1984; 132(5):2393-401. View

Trojanowski J, Hickey W . Human teratomas express differentiated neural antigens. An immunohistochemical study with anti-neurofilament, anti-glial filament, and anti-myelin basic protein monoclonal antibodies. Am J Pathol. 1984; 115(3):383-9. PMC: 1900511. View

Pappas C . CNS myelin and synapses in a spontaneous mouse ovarian teratoma showing neural differentiation. An immunohistochemical and electron microscopic study. J Neuropathol Exp Neurol. 1981; 40(3):289-97. DOI: 10.1097/00005072-198105000-00007. View

Nag S . Ultrastructural localization of lectin receptors on cerebral endothelium. Acta Neuropathol. 1985; 66(2):105-10. DOI: 10.1007/BF00688684. View

Hayes G, Woodroofe M, Cuzner M . Microglia are the major cell type expressing MHC class II in human white matter. J Neurol Sci. 1987; 80(1):25-37. DOI: 10.1016/0022-510x(87)90218-8. View

Kyritsis A, Tsokos M, Triche T, Chader G . Retinoblastoma--origin from a primitive neuroectodermal cell?. Nature. 1984; 307(5950):471-3. DOI: 10.1038/307471a0. View

Herman M, Sipe J, Rubinstein L, Vandenberg S, Spence A, VRAA-JENSEN J . An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. II. Electron microscopy. Am J Pathol. 1975; 81(2):421-44. PMC: 2032223. View

10.

KAHN H, Marks A, Thom H, Baumal R . Role of antibody to S100 protein in diagnostic pathology. Am J Clin Pathol. 1983; 79(3):341-7. DOI: 10.1093/ajcp/79.3.341. View

11.

Schelper R, ADRIAN Jr E . Monocytes become macrophages; they do not become microglia: a light and electron microscopic autoradiographic study using 125-iododeoxyuridine. J Neuropathol Exp Neurol. 1986; 45(1):1-19. DOI: 10.1097/00005072-198601000-00001. View

12.

Trojanowski J . Neurofilament proteins and human nervous system tumors. J Histochem Cytochem. 1987; 35(9):999-1003. DOI: 10.1177/35.9.3611738. View

13.

Giulian D, Baker T, Shih L, Lachman L . Interleukin 1 of the central nervous system is produced by ameboid microglia. J Exp Med. 1986; 164(2):594-604. PMC: 2188228. DOI: 10.1084/jem.164.2.594. View

14.

Vandenberg S, Herman M, Ludwin S, Bignami A . An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. I. Light microscopic and tissue and organ culture observations. Am J Pathol. 1975; 79(1):147-68. PMC: 1912641. View

15.

Vandenberg S, Hess J, Herman M, DeArmond S, Halks-Miller M, Rubinstein L . Neural differentiation in the OTT-6050 mouse teratoma. Production of a tumor fraction showing melanogenesis in neuroepithelial cells after centrifugal elutriation. Virchows Arch A Pathol Anat Histol. 1981; 392(3):295-308. DOI: 10.1007/BF02155667. View

16.

Pinkus G, ETHERIDGE C, OConnor E . Are keratin proteins a better tumor marker than epithelial membrane antigen? A comparative immunohistochemical study of various paraffin-embedded neoplasms using monoclonal and polyclonal antibodies. Am J Clin Pathol. 1986; 85(3):269-77. DOI: 10.1093/ajcp/85.3.269. View

17.

Miettinen M, Rapola J . Synaptophysin--an immuno-histochemical marker for childhood neuroblastoma. Acta Pathol Microbiol Immunol Scand A. 1987; 95(4):167-70. DOI: 10.1111/j.1699-0463.1987.tb00026_95a.x. View

18.

Gould V, Lee I, Wiedenmann B, Moll R, Chejfec G, Franke W . Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms. Hum Pathol. 1986; 17(10):979-83. DOI: 10.1016/s0046-8177(86)80080-6. View

19.

STERNBERGER L, Sternberger N . Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proc Natl Acad Sci U S A. 1983; 80(19):6126-30. PMC: 534374. DOI: 10.1073/pnas.80.19.6126. View

20.

Scott T . A rapid silver impregnation technique for oligodendrocytes, microglia, and astrocytes. J Clin Pathol. 1971; 24(6):578-80. PMC: 477095. DOI: 10.1136/jcp.24.6.578. View