Virulence Factors Enhance Citrobacter Rodentium Expansion Through Aerobic Respiration

Overview

Journal Science

Specialty Science

Date 2016 Sep 17

PMID 27634526

Citations 103

Authors

Christopher A Lopez

Brittany M Miller

Fabian Rivera-Chavez

Eric M Velazquez

Mariana X Byndloss

Alfredo Chavez-Arroyo

Kristen L Lokken

Renee M Tsolis

Sebastian E Winter

Andreas J Baumler

Affiliations

Soon will be listed here.

Abstract

Citrobacter rodentium uses a type III secretion system (T3SS) to induce colonic crypt hyperplasia in mice, thereby gaining an edge during its competition with the gut microbiota through an unknown mechanism. Here, we show that by triggering colonic crypt hyperplasia, the C. rodentium T3SS induced an excessive expansion of undifferentiated Ki67-positive epithelial cells, which increased oxygenation of the mucosal surface and drove an aerobic C. rodentium expansion in the colon. Treatment of mice with the γ-secretase inhibitor dibenzazepine to diminish Notch-driven colonic crypt hyperplasia curtailed the fitness advantage conferred by aerobic respiration during C. rodentium infection. We conclude that C. rodentium uses its T3SS to induce histopathological lesions that generate an intestinal microenvironment in which growth of the pathogen is fueled by aerobic respiration.

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