Heterologous Expression of PA8FAD9 and Functional Characterization of a Δ9-Fatty Acid Desaturase from a Cold-Tolerant Pseudomonas Sp. A8

Overview

Journal Mol Biotechnol

Publisher Springer

Specialties Biotechnology
Molecular Biology

Date 2016 Sep 16

PMID 27629791

Citations 3

Authors

Lawal Garba

Mohd Shukuri Mohamad Ali

Siti Nurbaya Oslan

Raja Noor Zaliha Raja Abdul Rahman

Affiliations

Soon will be listed here.

Abstract

Fatty acid desaturase enzymes are capable of inserting double bonds between carbon atoms of saturated fatty acyl-chains to produce unsaturated fatty acids. A gene coding for a putative Δ9-fatty acid desaturase-like protein was isolated from a cold-tolerant Pseudomonas sp. A8, cloned and heterologously expressed in Escherichia coli. The gene named as PA8FAD9 has an open reading frame of 1185 bp and codes for 394 amino acids with a predicted molecular weight of 45 kDa. The enzyme showed high Δ9-fatty acid desaturase-like protein activity and increased overall levels of cellular unsaturated fatty acids in the recombinant E. coli cells upon expression at different temperatures. The results showed that the ratio of palmitoleic to palmitic acid in the recombinant E. coli cells increased by more than twice the amount observed in the control cells at 20 °C using 0.4 mM IPTG. GCMS analysis confirmed the ability of this enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant E. coli membrane phospholipids. It may be concluded that the PA8FAD9 gene from Pseudomonas sp. A8 codes for a putative Δ9-fatty acid desaturase protein actively expressed in E. coli under the influence of temperature and an inducer.

Citing Articles

Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties.

Choi T, Park Y, Song H, Lee S, Park S, Lee H J Ind Microbiol Biotechnol. 2020; 47(12):1045-1057.

PMID: 33259029 DOI: 10.1007/s10295-020-02333-0.

Cold Tolerance Regulated by the Pyruvate Metabolism in .

Xie T, Pang R, Wu Q, Zhang J, Lei T, Li Y Front Microbiol. 2019; 10:178.

PMID: 30787922 PMC: 6372572. DOI: 10.3389/fmicb.2019.00178.

Homology modeling and docking studies of a Δ9-fatty acid desaturase from a Cold-tolerant sp. AMS8.

Garba L, Mohamad Yussoff M, Abd Halim K, Ishak S, Mohamad Ali M, Oslan S PeerJ. 2018; 6:e4347.

PMID: 29576935 PMC: 5863719. DOI: 10.7717/peerj.4347.

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