Comparison of Osteogenesis Between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactone/β-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2016 Sep 10

PMID 27610141

Citations 15

Authors

Yongsun Kim

Seung Hoon Lee

Byung-Jae Kang

Wan Hee Kim

Hui-Suk Yun

Oh-Kyeong Kweon

Affiliations

Soon will be listed here.

Abstract

Multipotent mesenchymal stem cells (MSCs) and MSC sheets have effective potentials of bone regeneration. Composite polymer/ceramic scaffolds such as poly-ε-caprolactone (PCL)/β-tricalcium phosphate (β-TCP) are widely used to repair large bone defects. The present study investigated the in vitro osteogenic potential of canine adipose-derived MSCs (Ad-MSCs) and Ad-MSC sheets. Composite PCL/β-TCP scaffolds seeded with Ad-MSCs or wrapped with osteogenic Ad-MSC sheets (OCS) were also fabricated and their osteogenic potential was assessed following transplantation into critical-sized bone defects in dogs. The alkaline phosphatase (ALP) activity of osteogenic Ad-MSCs (O-MSCs) and OCS was significantly higher than that of undifferentiated Ad-MSCs (U-MSCs). The ALP, runt-related transcription factor 2, osteopontin, and bone morphogenetic protein 7 mRNA levels were upregulated in O-MSCs and OCS as compared to U-MSCs. In a segmental bone defect, the amount of newly formed bone was greater in PCL/β-TCP/OCS and PCL/β-TCP/O-MSCs/OCS than in the other groups. The OCS exhibit strong osteogenic capacity, and OCS combined with a PCL/β-TCP composite scaffold stimulated new bone formation in a critical-sized bone defect. These results suggest that the PCL/β-TCP/OCS composite has potential clinical applications in bone regeneration and can be used as an alternative treatment modality in bone tissue engineering.

Citing Articles

Current status of nano-embedded growth factors and stem cells delivery to bone for targeted repair and regeneration.

Liang W, Zhou C, Liu X, Xie Q, Xia L, Liu L J Orthop Translat. 2025; 50:257-273.

PMID: 39902262 PMC: 11788687. DOI: 10.1016/j.jot.2024.12.006.

Stem Cells and Bone Tissue Engineering.

Gao X, Ruzbarsky J, Layne J, Xiao X, Huard J Life (Basel). 2024; 14(3).

PMID: 38541613 PMC: 10971350. DOI: 10.3390/life14030287.

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals.

Ferreira-Baptista C, Ferreira R, Fernandes M, Gomes P, Colaco B Vet Sci. 2023; 10(12).

PMID: 38133224 PMC: 10747344. DOI: 10.3390/vetsci10120673.

Recent advances on 3D-printed PCL-based composite scaffolds for bone tissue engineering.

Gharibshahian M, Salehi M, Beheshtizadeh N, Kamalabadi-Farahani M, Atashi A, Nourbakhsh M Front Bioeng Biotechnol. 2023; 11:1168504.

PMID: 37469447 PMC: 10353441. DOI: 10.3389/fbioe.2023.1168504.

Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.

Banimohamad-Shotorbani B, Karkan S, Rahbarghazi R, Mehdipour A, Jarolmasjed S, Saghati S Stem Cell Res Ther. 2023; 14(1):68.

PMID: 37024981 PMC: 10080954. DOI: 10.1186/s13287-023-03309-4.

References

Simpson R, Wiria F, Amis A, Chua C, Leong K, Hansen U . Development of a 95/5 poly(L-lactide-co-glycolide)/hydroxylapatite and beta-tricalcium phosphate scaffold as bone replacement material via selective laser sintering. J Biomed Mater Res B Appl Biomater. 2007; 84(1):17-25. DOI: 10.1002/jbm.b.30839. View

Vanhatupa S, Ojansivu M, Autio R, Juntunen M, Miettinen S . Bone Morphogenetic Protein-2 Induces Donor-Dependent Osteogenic and Adipogenic Differentiation in Human Adipose Stem Cells. Stem Cells Transl Med. 2015; 4(12):1391-402. PMC: 4675505. DOI: 10.5966/sctm.2015-0042. View

Chen G, Deng C, Li Y . TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 2012; 8(2):272-88. PMC: 3269610. DOI: 10.7150/ijbs.2929. View

Ryu H, Lim J, Byeon Y, Park J, Seo M, Lee Y . Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury. J Vet Sci. 2009; 10(4):273-84. PMC: 2807262. DOI: 10.4142/jvs.2009.10.4.273. View

Vieira N, Brandalise V, Zucconi E, Secco M, Strauss B, Zatz M . Isolation, characterization, and differentiation potential of canine adipose-derived stem cells. Cell Transplant. 2009; 19(3):279-89. DOI: 10.3727/096368909X481764. View

Akahane M, Nakamura A, Ohgushi H, Shigematsu H, Dohi Y, Takakura Y . Osteogenic matrix sheet-cell transplantation using osteoblastic cell sheet resulted in bone formation without scaffold at an ectopic site. J Tissue Eng Regen Med. 2008; 2(4):196-201. DOI: 10.1002/term.81. View

Pensak M, Hong S, Dukas A, Tinsley B, Drissi H, Tang A . The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur. Gene Ther. 2015; 22(6):467-75. DOI: 10.1038/gt.2015.14. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Muller P, Bulnheim U, Diener A, Luthen F, Teller M, Klinkenberg E . Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells. J Cell Mol Med. 2008; 12(1):281-91. PMC: 3823489. DOI: 10.1111/j.1582-4934.2007.00103.x. View

10.

Marino G, Rosso F, Cafiero G, Tortora C, Moraci M, Barbarisi M . Beta-tricalcium phosphate 3D scaffold promote alone osteogenic differentiation of human adipose stem cells: in vitro study. J Mater Sci Mater Med. 2009; 21(1):353-63. DOI: 10.1007/s10856-009-3840-z. View

11.

Xie Q, Wang Z, Huang Y, Bi X, Zhou H, Lin M . Characterization of human ethmoid sinus mucosa derived mesenchymal stem cells (hESMSCs) and the application of hESMSCs cell sheets in bone regeneration. Biomaterials. 2015; 66:67-82. DOI: 10.1016/j.biomaterials.2015.07.013. View

12.

Kang B, Ryu H, Park S, Koyama Y, Kikuchi M, Woo H . Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects. J Vet Sci. 2012; 13(3):299-310. PMC: 3467406. DOI: 10.4142/jvs.2012.13.3.299. View

13.

Neupane M, Chang C, Kiupel M, Yuzbasiyan-Gurkan V . Isolation and characterization of canine adipose-derived mesenchymal stem cells. Tissue Eng Part A. 2009; 14(6):1007-15. DOI: 10.1089/ten.tea.2007.0207. View

14.

Yu J, Tu Y, Tang Y, Cheng N . Stemness and transdifferentiation of adipose-derived stem cells using L-ascorbic acid 2-phosphate-induced cell sheet formation. Biomaterials. 2014; 35(11):3516-26. DOI: 10.1016/j.biomaterials.2014.01.015. View

15.

Long T, Zhu Z, Awad H, Schwarz E, Hilton M, Dong Y . The effect of mesenchymal stem cell sheets on structural allograft healing of critical sized femoral defects in mice. Biomaterials. 2014; 35(9):2752-9. PMC: 3913373. DOI: 10.1016/j.biomaterials.2013.12.039. View

16.

Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X . Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration. PLoS One. 2015; 10(5):e0125253. PMC: 4430281. DOI: 10.1371/journal.pone.0125253. View

17.

Arinzeh T, Peter S, Archambault M, van den Bos C, Gordon S, Kraus K . Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect. J Bone Joint Surg Am. 2003; 85(10):1927-35. DOI: 10.2106/00004623-200310000-00010. View

18.

Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N . Bone formation and resorption of highly purified beta-tricalcium phosphate in the rat femoral condyle. Biomaterials. 2005; 26(28):5600-8. DOI: 10.1016/j.biomaterials.2005.02.026. View

19.

Akahane M, Shigematsu H, Tadokoro M, Ueha T, Matsumoto T, Tohma Y . Scaffold-free cell sheet injection results in bone formation. J Tissue Eng Regen Med. 2010; 4(5):404-11. DOI: 10.1002/term.259. View

20.

Lai R, Tan S, Teh B, Sze S, Arslan F, de Kleijn D . Proteolytic Potential of the MSC Exosome Proteome: Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome. Int J Proteomics. 2012; 2012:971907. PMC: 3407643. DOI: 10.1155/2012/971907. View