Molecular Characterization and Sequence Analysis of Echinococcus Granulosus from Sheep Isolates in East Azerbaijan Province, Northwest of Iran

Overview

Journal J Parasit Dis

Specialty Parasitology

Date 2016 Sep 9

PMID 27605785

Citations 7

Authors

Mahmoud Mahami Oskouei

Nader Ghabouli Mehrabani

Abolfazl Miahipour

Esmaeil Fallah

Affiliations

Soon will be listed here.

Abstract

Echinococcus granulosus as an etiologic agent of hydatid cyst is one of the most important zoonotic helminthes in the world that causing enormous economic and health losses. The aim of this study was to evaluate genotype of E. granulosus isolated from sheep using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and sequencing method in East Azerbaijan province, northwest of Iran. Nineteen sheep hydatid cyst samples were collected. Genomic DNA was extracted from protoscoleces using commercial DNA extraction kit. Mitochondrial cox1 region was amplified by polymerase chain reaction (PCR) and all isolates were sequenced. Afterward, sequences were analyzed for determination of genotypes by related software. G1 (94.73 %) and G3 (5.27 %) genotypes were identified from the isolates which out of 19 hydatid cysts, 17 samples were G1B, 1 sample G1D and the other one had G3 genotype. Results of this study indicate that common sheep strain (G1); especially G1B is the dominant subtype of E. granulosus in East Azerbaijan province.

Citing Articles

Prevalence of Cystic Echinococcosis Genotypes in Iranian Animals: A Systematic Review and Meta-Analysis.

Khodashenas S, Akbari M, Beiranvand R, Didehdar M, Shabani M, Iravani P J Parasitol Res. 2022; 2022:8197741.

PMID: 36312205 PMC: 9605837. DOI: 10.1155/2022/8197741.

Genetic diversity of spp. in wild carnivorous animals in Kazakhstan.

Uakhit R, Smagulova A, Syzdykova A, Abdrakhmanov S, Kiyan V Vet World. 2022; 15(6):1489-1496.

PMID: 35993082 PMC: 9375211. DOI: 10.14202/vetworld.2022.1489-1496.

Therapeutic efficacy of nanocompounds in the treatment of cystic and alveolar echinococcoses: challenges and future prospects.

Bakhtiar N, Akbarzadeh A, Casulli A, Mahami-Oskouei M, Ahmadpour E, Nami S Parasitol Res. 2019; 118(9):2455-2466.

PMID: 31402401 DOI: 10.1007/s00436-019-06416-5.

Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China.

Guo B, Zhang Z, Zheng X, Guo Y, Guo G, Zhao L Korean J Parasitol. 2019; 57(2):153-159.

PMID: 31104407 PMC: 6526214. DOI: 10.3347/kjp.2019.57.2.153.

The potential role of toll-like receptor 4 Asp299Gly polymorphism and its association with recurrent cystic echinococcosis in postoperative patients.

Noori J, Spotin A, Ahmadpour E, Mahami-Oskouei M, Sadeghi-Bazargani H, Kazemi T Parasitol Res. 2018; 117(6):1717-1727.

PMID: 29602972 DOI: 10.1007/s00436-018-5850-6.

References

Varcasia A, Canu S, Kogkos A, Pipia A, Scala A, Garippa G . Molecular characterization of Echinococcus granulosus in sheep and goats of Peloponnesus, Greece. Parasitol Res. 2007; 101(4):1135-9. DOI: 10.1007/s00436-007-0568-x. View

Bart J, Abdukader M, Zhang Y, Lin R, Wang Y, Nakao M . Genotyping of human cystic echinococcosis in Xinjiang, PR China. Parasitology. 2006; 133(Pt 5):571-9. DOI: 10.1017/S0031182006000734. View

Pour A, Hosseini S, Shayan P . Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res. 2010; 108(5):1229-34. DOI: 10.1007/s00436-010-2170-x. View

Monteiro D, Botton S, Tonin A, Azevedo M, Graichen D, Noal C . Echinococcus canadensis (G7) and Echinococcus granulosus sensu stricto (G1) in swine of southern Brazil. Vet Parasitol. 2014; 202(3-4):335-8. DOI: 10.1016/j.vetpar.2014.01.023. View

Beato S, Parreira R, Calado M, Gracio M . Apparent dominance of the G1-G3 genetic cluster of Echinococcus granulosus strains in the central inland region of Portugal. Parasitol Int. 2010; 59(4):638-42. DOI: 10.1016/j.parint.2010.08.004. View

Moro P, Schantz P . Echinococcosis: a review. Int J Infect Dis. 2008; 13(2):125-33. DOI: 10.1016/j.ijid.2008.03.037. View

Mrad S, Filisetti D, Oudni M, Mekki M, Belguith M, Nouri A . Molecular evidence of ovine (G1) and camel (G6) strains of Echinococcus granulosus in Tunisia and putative role of cattle in human contamination. Vet Parasitol. 2005; 129(3-4):267-72. DOI: 10.1016/j.vetpar.2005.02.006. View

Bowles J, McManus D . NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol. 1993; 23(7):969-72. DOI: 10.1016/0020-7519(93)90065-7. View

Adwan G, Adwan K, Bdir S, Abuseir S . Molecular characterization of Echinococcus granulosus isolated from sheep in Palestine. Exp Parasitol. 2013; 134(2):195-9. DOI: 10.1016/j.exppara.2013.03.024. View

10.

Jabbar A, Narankhajid M, Nolan M, Jex A, Campbell B, Gasser R . A first insight into the genotypes of Echinococcus granulosus from humans in Mongolia. Mol Cell Probes. 2010; 25(1):49-54. DOI: 10.1016/j.mcp.2010.11.001. View

11.

Ma S, Maillard S, Zhao H, Huang X, Wang H, Geng P . Assessment of Echinococcus granulosus polymorphism in Qinghai province, People's Republic of China. Parasitol Res. 2008; 102(6):1201-6. DOI: 10.1007/s00436-008-0894-7. View

12.

Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A . Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): an inference from nuclear protein-coding genes. Mol Phylogenet Evol. 2011; 61(3):628-38. DOI: 10.1016/j.ympev.2011.07.022. View

13.

Vural G, Baca A, Gauci C, Bagci O, Gicik Y, Lightowlers M . Variability in the Echinococcus granulosus cytochrome C oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol. 2008; 154(3-4):347-50. DOI: 10.1016/j.vetpar.2008.03.020. View

14.

Boufana B, Stidworthy M, Bell S, Chantrey J, Masters N, Unwin S . Echinococcus and Taenia spp. from captive mammals in the United Kingdom. Vet Parasitol. 2012; 190(1-2):95-103. DOI: 10.1016/j.vetpar.2012.05.023. View

15.

Varcasia A, Canu S, Lightowlers M, Scala A, Garippa G . Molecular characterization of Echinococcus granulosus strains in Sardinia. Parasitol Res. 2005; 98(3):273-7. DOI: 10.1007/s00436-005-0059-x. View

16.

Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M . Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2012; 87(4):387-91. DOI: 10.1017/S0022149X1200051X. View

17.

Konyaev S, Yanagida T, Nakao M, Ingovatova G, Shoykhet Y, Bondarev A . Genetic diversity of Echinococcus spp. in Russia. Parasitology. 2013; 140(13):1637-47. DOI: 10.1017/S0031182013001340. View

18.

Hajialilo E, Harandi M, Sharbatkhori M, Mirhendi H, Rostami S . Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol. 2011; 86(3):263-70. DOI: 10.1017/S0022149X11000320. View

19.

Sharma M, Sehgal R, Fomda B, Malhotra A, Malla N . Molecular characterization of Echinococcus granulosus cysts in north Indian patients: identification of G1, G3, G5 and G6 genotypes. PLoS Negl Trop Dis. 2013; 7(6):e2262. PMC: 3681628. DOI: 10.1371/journal.pntd.0002262. View

20.

Thompson R . The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119(4):439-446. DOI: 10.1016/j.exppara.2008.04.016. View