The Myosin X Motor is Optimized for Movement on Actin Bundles

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Sep 2

PMID 27580874

Citations 42

Authors

Virginie Ropars

Zhaohui Yang

Tatiana Isabet

Florian Blanc

Kaifeng Zhou

Tianming Lin

Xiaoyan Liu

Pascale Hissier

Frederic Samazan

Beatrice Amigues

Eric D Yang

Hyokeun Park

Olena Pylypenko

Marco Cecchini

Charles V Sindelar

H Lee Sweeney

Anne Houdusse

Affiliations

Soon will be listed here.

Abstract

Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components of this motor, and characterizing the in vitro behaviour of the native dimer, we identify the features that explain the myosin X dimer behaviour. Single-molecule studies demonstrate that a native myosin X dimer moves on actin bundles with higher velocities and takes larger steps than on single actin filaments. The largest steps on actin bundles are larger than previously reported for artificially dimerized myosin X constructs or any other myosin. Our model and kinetic data explain why these large steps and high velocities can only occur on bundled filaments. Thus, myosin X functions as an antiparallel dimer in cells with a unique geometry optimized for movement on actin bundles.

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