Cell-sized Asymmetric Lipid Vesicles Facilitate the Investigation of Asymmetric Membranes

Overview

Journal Nat Chem

Specialty Chemistry

Date 2016 Aug 25

PMID 27554415

Citations 45

Authors

Koki Kamiya

Ryuji Kawano

Toshihisa Osaki

Kazunari Akiyoshi

Shoji Takeuchi

Affiliations

Soon will be listed here.

Abstract

Asymmetric lipid giant vesicles have been used to model the biochemical reactions in cell membranes. However, methods for producing asymmetric giant vesicles lead to the inclusion of an organic solvent layer that affects the mechanical and physical characteristics of the membrane. Here we describe the formation of asymmetric giant vesicles that include little organic solvent, and use them to investigate the dynamic responses of lipid molecules in the vesicle membrane. We formed the giant vesicles via the inhomogeneous break-up of a lipid microtube generated by applying a jet flow to an asymmetric planar lipid bilayer. The asymmetric giant vesicles showed a lipid flip-flop behaviour in the membrane, superficially similar to the lipid flip-flop activity observed in apoptotic cells. In vitro synthesis of membrane proteins into the asymmetric giant vesicles revealed that the lipid asymmetry in bilayer membranes improves the reconstitution ratio of membrane proteins. Our asymmetric giant vesicles will be useful in elucidating lipid-lipid and lipid-membrane protein interactions involved in the regulation of cellular functions.

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