An Ultrasensitive Electrogenerated Chemiluminescence-based Immunoassay for Specific Detection of Zika Virus

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 25

PMID 27554037

Citations 21

Authors

Dhiraj Acharya

Pradip Bastola

Linda Le

Amber M Paul

Estefania Fernandez

Michael S Diamond

Wujian Miao

Fengwei Bai

Affiliations

Soon will be listed here.

Abstract

Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research.

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References

DOrtenzio E, Matheron S, Yazdanpanah Y, de Lamballerie X, Hubert B, Piorkowski G . Evidence of Sexual Transmission of Zika Virus. N Engl J Med. 2016; 374(22):2195-8. DOI: 10.1056/NEJMc1604449. View

Chowdhury F, Williams A, Johnson P . Validation and comparison of two multiplex technologies, Luminex and Mesoscale Discovery, for human cytokine profiling. J Immunol Methods. 2008; 340(1):55-64. DOI: 10.1016/j.jim.2008.10.002. View

Miao W . Electrogenerated chemiluminescence and its biorelated applications. Chem Rev. 2008; 108(7):2506-53. DOI: 10.1021/cr068083a. View

Parajuli S, Miao W . Sensitive determination of hexamethylene triperoxide diamine explosives, using electrogenerated chemiluminescence enhanced by silver nitrate. Anal Chem. 2009; 81(13):5267-72. DOI: 10.1021/ac900489a. View

Shuaib W, Stanazai H, Abazid A, Mattar A . Re-Emergence of Zika Virus: A Review on Pathogenesis, Clinical Manifestations, Diagnosis, Treatment, and Prevention. Am J Med. 2016; 129(8):879.e7-879.e12. DOI: 10.1016/j.amjmed.2016.02.027. View

Armstrong P, Hennessey M, Adams M, Cherry C, Chiu S, Harrist A . Travel-Associated Zika Virus Disease Cases Among U.S. Residents--United States, January 2015-February 2016. MMWR Morb Mortal Wkly Rep. 2016; 65(11):286-9. DOI: 10.15585/mmwr.mm6511e1. View

Deckard D, Chung W, Brooks J, Smith J, Woldai S, Hennessey M . Male-to-Male Sexual Transmission of Zika Virus--Texas, January 2016. MMWR Morb Mortal Wkly Rep. 2016; 65(14):372-4. DOI: 10.15585/mmwr.mm6514a3. View

Sun B, Qi H, Ma F, Gao Q, Zhang C, Miao W . Double covalent coupling method for the fabrication of highly sensitive and reusable electrogenerated chemiluminescence sensors. Anal Chem. 2010; 82(12):5046-52. DOI: 10.1021/ac9029289. View

Rabenau H, Kessler H, Kortenbusch M, Steinhorst A, Raggam R, Berger A . Verification and validation of diagnostic laboratory tests in clinical virology. J Clin Virol. 2007; 40(2):93-8. DOI: 10.1016/j.jcv.2007.07.009. View

10.

Paty M . [The expansion of vector-borne diseases and the implications for blood transfusion safety: The case of West Nile Virus, dengue and chikungunya]. Transfus Clin Biol. 2013; 20(2):165-73. DOI: 10.1016/j.tracli.2013.03.008. View

11.

Ma F, Zhang Y, Qi H, Gao Q, Zhang C, Miao W . Ultrasensitive electrogenerated chemiluminescence biosensor for the determination of mercury ion incorporating G4 PAMAM dendrimer and Hg(II)-specific oligonucleotide. Biosens Bioelectron. 2012; 32(1):37-42. DOI: 10.1016/j.bios.2011.11.011. View

12.

Weaver S, Reisen W . Present and future arboviral threats. Antiviral Res. 2009; 85(2):328-45. PMC: 2815176. DOI: 10.1016/j.antiviral.2009.10.008. View

13.

Cerbino-Neto J, Mesquita E, Souza T, Parreira V, Wittlin B, Durovni B . Clinical Manifestations of Zika Virus Infection, Rio de Janeiro, Brazil, 2015. Emerg Infect Dis. 2016; 22(7):1318-20. PMC: 4918188. DOI: 10.3201/eid2207.160375. View

14.

Miao W, Bard A . Electrogenerated chemiluminescence. 80. C-reactive protein determination at high amplification with [Ru(bpy)3]2+-containing microspheres. Anal Chem. 2004; 76(23):7109-13. DOI: 10.1021/ac048782s. View

15.

Balm M, Lee C, Lee H, Chiu L, Koay E, Tang J . A diagnostic polymerase chain reaction assay for Zika virus. J Med Virol. 2012; 84(9):1501-5. DOI: 10.1002/jmv.23241. View

16.

Doeven E, Barbante G, Kerr E, Hogan C, Endler J, Francis P . Red-green-blue electrogenerated chemiluminescence utilizing a digital camera as detector. Anal Chem. 2014; 86(5):2727-32. DOI: 10.1021/ac404135f. View

17.

Koraka P, Zeller H, Niedrig M, Osterhaus A, Groen J . Reactivity of serum samples from patients with a flavivirus infection measured by immunofluorescence assay and ELISA. Microbes Infect. 2002; 4(12):1209-15. DOI: 10.1016/s1286-4579(02)01647-7. View

18.

Kilianski A, Roth P, Liem A, Hill J, Willis K, Rossmaier R . Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses. Emerg Infect Dis. 2016; 22(8):1448-51. PMC: 4982148. DOI: 10.3201/eid2208.160270. View

19.

Rivera V, Gamez F, Keener W, White J, Poli M . Rapid detection of Clostridium botulinum toxins A, B, E, and F in clinical samples, selected food matrices, and buffer using paramagnetic bead-based electrochemiluminescence detection. Anal Biochem. 2006; 353(2):248-56. DOI: 10.1016/j.ab.2006.02.030. View

20.

Li J, Xiong Y, Wu W, Liu X, Qu J, Zhao X . Zika Virus in a Traveler Returning to China from Caracas, Venezuela, February 2016. Emerg Infect Dis. 2016; 22(6):1133-6. PMC: 4880083. DOI: 10.3201/eid2206.160273. View