Enterovirus 71 Virus Propagation and Purification

Overview

Journal Bio Protoc

Specialty Biology

Date 2016 Aug 23

PMID 27547789

Citations 5

Authors

Kristin L Shingler

Lindsey J Organtini

Susan Hafenstein

Affiliations

Soon will be listed here.

Abstract

Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause severe disease and death. This is the propagation and purification procedure to produce infectious virion.

Citing Articles

A tradeoff between enterovirus A71 particle stability and cell entry.

Catching A, Yeh M, Bianco S, Capponi S, Andino R Nat Commun. 2023; 14(1):7450.

PMID: 37978288 PMC: 10656440. DOI: 10.1038/s41467-023-43029-0.

A human monoclonal antibody binds within the poliovirus receptor-binding site to neutralize all three serotypes.

Charnesky A, Faust J, Lee H, Puligedda R, Goetschius D, DiNunno N Nat Commun. 2023; 14(1):6335.

PMID: 37816742 PMC: 10564760. DOI: 10.1038/s41467-023-41052-9.

Genomic analysis of serologically untypable human enteroviruses in Taiwan.

Chien Y, Luo S, Tsao K, Huang Y, Chung W, Liao Y J Biomed Sci. 2019; 26(1):49.

PMID: 31266491 PMC: 6607526. DOI: 10.1186/s12929-019-0541-x.

Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus-A71 capsid.

Sun L, Lee H, Thibaut H, Lanko K, Rivero-Buceta E, Bator C PLoS Pathog. 2019; 15(5):e1007760.

PMID: 31071193 PMC: 6590834. DOI: 10.1371/journal.ppat.1007760.

Honey Bee Deformed Wing Virus Structures Reveal that Conformational Changes Accompany Genome Release.

Organtini L, Shingler K, Ashley R, Capaldi E, Durrani K, Dryden K J Virol. 2016; 91(2).

PMID: 27852845 PMC: 5215330. DOI: 10.1128/JVI.01795-16.

References

Shingler K, Yoder J, Carnegie M, Ashley R, Makhov A, Conway J . The enterovirus 71 A-particle forms a gateway to allow genome release: a cryoEM study of picornavirus uncoating. PLoS Pathog. 2013; 9(3):e1003240. PMC: 3605244. DOI: 10.1371/journal.ppat.1003240. View

Cifuente J, Lee H, Yoder J, Shingler K, Carnegie M, Yoder J . Structures of the procapsid and mature virion of enterovirus 71 strain 1095. J Virol. 2013; 87(13):7637-45. PMC: 3700288. DOI: 10.1128/JVI.03519-12. View