Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2016 Aug 23

PMID 27545885

Citations 67

Authors

Samuele Calabro

Dong Liu

Antonia Gallman

Manuela Sales L Nascimento

Zizi Yu

Ting-Ting Zhang

Pei Chen

Biyan Zhang

Lan Xu

Uthaman Gowthaman

Jayendra Kumar Krishnaswamy

Ann M Haberman

Adam Williams

Stephanie C Eisenbarth

Affiliations

Soon will be listed here.

Abstract

Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens.

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