Two-photon Excited Fluorescence of Intrinsic Fluorophores Enables Label-free Assessment of Adipose Tissue Function

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 6

PMID 27491409

Citations 20

Authors

Carlo Amadeo Alonzo

Sevasti Karaliota

Dimitra Pouli

Zhiyi Liu

Katia P Karalis

Irene Georgakoudi

Affiliations

Soon will be listed here.

Abstract

Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imaging of adipocyte metabolism with subcellular resolution. Steady-state and time-resolved fluorescence from intracellular metabolic co-factors and lipid droplets can distinguish the functional states of excised white, brown, and cold-induced beige fat. Similar optical changes are identified when white and brown fat are assessed in vivo. Therefore, these studies establish the potential of non-invasive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue types, monitor their functional state, and characterize heterogeneity of induced responses.

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