Molecular Self-assembly Using Peptide Nucleic Acids

Overview

Journal Biopolymers

Publisher Wiley

Date 2016 Aug 4

PMID 27486924

Citations 13

Authors

Or Berger

Ehud Gazit

Affiliations

Soon will be listed here.

Abstract

Peptide nucleic acids (PNAs) are extensively studied for the control of genetic expression since their design in the 1990s. However, the application of PNAs in nanotechnology is much more recent. PNAs share the specific base-pair recognition characteristic of DNA together with material-like properties of polyamides, both proteins and synthetic polymers, such as Kevlar and Nylon. The first application of PNA was in the form of PNA-amphiphiles, resulting in the formation of either lipid integrated structures, hydrogels or fibrillary assemblies. Heteroduplex DNA-PNA assemblies allow the formation of hybrid structures with higher stability as compared with pure DNA. A systematic screen for minimal PNA building blocks resulted in the identification of guanine-containing di-PNA assemblies and protected guanine-PNA monomer spheres showing unique optical properties. Finally, the co-assembly of PNA with thymine-like three-faced cyanuric acid allowed the assembly of poly-adenine PNA into fibers. In summary, we believe that PNAs represent a new and important family of building blocks which converges the advantages of both DNA- and peptide-nanotechnologies.

Citing Articles

Tryptophan-PNA gc Conjugates Self-Assemble to Form Fibers.

Mosseri A, Sancho-Albero M, Mercurio F, Leone M, De Cola L, Romanelli A Bioconjug Chem. 2023; 34(8):1429-1438.

PMID: 37486977 PMC: 10436247. DOI: 10.1021/acs.bioconjchem.3c00200.

Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis.

Zhao M, Wang R, Yang K, Jiang Y, Peng Y, Li Y Acta Pharm Sin B. 2023; 13(3):916-941.

PMID: 36970219 PMC: 10031267. DOI: 10.1016/j.apsb.2022.10.019.

Intrinsic fluorescence of nucleobase crystals.

Aizen R, Arnon Z, Berger O, Ruggiero A, Zaguri D, Brown N Nanoscale Adv. 2023; 5(2):344-348.

PMID: 36756258 PMC: 9846435. DOI: 10.1039/d2na00551d.

Solvent modulation in peptide sub-microfibers obtained by solution blow spinning.

Dias A, Cena C, Lutz-Bueno V, Mezzenga R, Marques A, Ferreira I Front Chem. 2022; 10:1054347.

PMID: 36561144 PMC: 9763608. DOI: 10.3389/fchem.2022.1054347.

Artificial genetic polymers against human pathologies.

Ivanov G, Tribulovich V, Pestov N, David T, Amoah A, Korneenko T Biol Direct. 2022; 17(1):39.

PMID: 36474260 PMC: 9727881. DOI: 10.1186/s13062-022-00353-7.

References

Dietz H, Douglas S, Shih W . Folding DNA into twisted and curved nanoscale shapes. Science. 2009; 325(5941):725-30. PMC: 2737683. DOI: 10.1126/science.1174251. View

Ray A, Norden B . Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future. FASEB J. 2000; 14(9):1041-60. DOI: 10.1096/fasebj.14.9.1041. View

Cui H, Webber M, Stupp S . Self-assembly of peptide amphiphiles: from molecules to nanostructures to biomaterials. Biopolymers. 2010; 94(1):1-18. PMC: 2921868. DOI: 10.1002/bip.21328. View

Seeman N . Structural DNA nanotechnology: growing along with Nano Letters. Nano Lett. 2010; 10(6):1971-8. PMC: 2901229. DOI: 10.1021/nl101262u. View

Berger O, Adler-Abramovich L, Levy-Sakin M, Grunwald A, Liebes-Peer Y, Bachar M . Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson-Crick base pairing. Nat Nanotechnol. 2015; 10(4):353-60. DOI: 10.1038/nnano.2015.27. View

Nielsen P, Egholm M, Buchardt O . Peptide nucleic acid (PNA). A DNA mimic with a peptide backbone. Bioconjug Chem. 1994; 5(1):3-7. DOI: 10.1021/bc00025a001. View

Seeman N . An overview of structural DNA nanotechnology. Mol Biotechnol. 2007; 37(3):246-57. PMC: 3479651. DOI: 10.1007/s12033-007-0059-4. View

Vernille J, Kovell L, Schneider J . Peptide nucleic acid (PNA) amphiphiles: synthesis, self-assembly, and duplex stability. Bioconjug Chem. 2004; 15(6):1314-21. DOI: 10.1021/bc049831a. View

Pinheiro A, Han D, Shih W, Yan H . Challenges and opportunities for structural DNA nanotechnology. Nat Nanotechnol. 2011; 6(12):763-72. PMC: 3334823. DOI: 10.1038/nnano.2011.187. View

10.

Seeman N . DNA nanotechnology: novel DNA constructions. Annu Rev Biophys Biomol Struct. 1998; 27:225-48. DOI: 10.1146/annurev.biophys.27.1.225. View

11.

Douglas S, Dietz H, Liedl T, Hogberg B, Graf F, Shih W . Self-assembly of DNA into nanoscale three-dimensional shapes. Nature. 2009; 459(7245):414-8. PMC: 2688462. DOI: 10.1038/nature08016. View

12.

Bonifazi D, Carloni L, Corvaglia V, Delforge A . Peptide nucleic acids in materials science. Artif DNA PNA XNA. 2012; 3(3):112-22. PMC: 3581510. DOI: 10.4161/adna.21941. View

13.

Egholm M, Buchardt O, Christensen L, Behrens C, Freier S, Driver D . PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. Nature. 1993; 365(6446):566-8. DOI: 10.1038/365566a0. View

14.

Davis J, Spada G . Supramolecular architectures generated by self-assembly of guanosine derivatives. Chem Soc Rev. 2007; 36(2):296-313. DOI: 10.1039/b600282j. View

15.

Williams K, Veenhuizen P, de la Torre B, Eritja R, Dekker C . Nanotechnology: carbon nanotubes with DNA recognition. Nature. 2002; 420(6917):761. DOI: 10.1038/420761a. View

16.

Datta B, Bier M, Roy S, Armitage B . Quadruplex formation by a guanine-rich PNA oligomer. J Am Chem Soc. 2005; 127(12):4199-207. DOI: 10.1021/ja0446202. View

17.

Nielsen P, Egholm M, Berg R, Buchardt O . Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science. 1991; 254(5037):1497-500. DOI: 10.1126/science.1962210. View

18.

Guler M, Pokorski J, Appella D, Stupp S . Enhanced oligonucleotide binding to self-assembled nanofibers. Bioconjug Chem. 2005; 16(3):501-3. DOI: 10.1021/bc050053b. View

19.

Wittung P, Nielsen P, Buchardt O, Egholm M, Norden B . DNA-like double helix formed by peptide nucleic acid. Nature. 1994; 368(6471):561-3. DOI: 10.1038/368561a0. View

20.

Flory J, Simmons C, Lin S, Johnson T, Andreoni A, Zook J . Low temperature assembly of functional 3D DNA-PNA-protein complexes. J Am Chem Soc. 2014; 136(23):8283-95. DOI: 10.1021/ja501228c. View