Age-dependent Modifications of Mitochondrial Proteins in Cerebral Cortex and Striatum of Rat Brain

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1989 May 1

PMID 2747833

Citations 6

Authors

N Ragusa

L Turpeenoja

G Magri

P Lahdesmaki

A M Giuffrida Stella

Affiliations

Soon will be listed here.

Abstract

The protein composition of free mitochondria purified from cerebral cortex and striatum during aging was analyzed by gel electrophoresis. Mitochondria were isolated from cerebral cortex and striatum of 4-, 12-, and 24-month-old rat brain. The percent amount of mitochondrial proteins after gel-electrophoretic separation was determined densitometrically. A significant decrease in the amount of two polypeptides (with molecular weights of 20 and 16 kDa, respectively) in both brain regions during aging was found. The decrease was higher in the striatum indicating a greater vulnerability of this brain area to the aging process. The age-dependent modifications of mitochondrial proteins observed may play an important role in several mitochondrial functions, such as energy transduction and transport processes as well as in structural changes occurring with age, causing altered membrane permeability and fluidity.

Citing Articles

Effect of in vivo L-acetylcarnitine administration on ATP-ases enzyme systems of synaptic plasma membranes from rat cerebral cortex.

Villa R, Ferrari F, Gorini A Neurochem Res. 2011; 36(8):1372-82.

PMID: 21479591 DOI: 10.1007/s11064-011-0462-x.

Cerebral cortex synaptic heavy mitochondria may represent the oldest synaptic mitochondrial population: biochemical heterogeneity and effects of L-acetylcarnitine.

Battino M, Quiles J, Huertas J, Mataix J, Villa R, Gorini A J Bioenerg Biomembr. 2002; 32(2):163-73.

PMID: 11768749 DOI: 10.1023/a:1005559930210.

Action of L-acetylcarnitine on different cerebral mitochondrial populations from cerebral cortex.

Gorini A, DAngelo A, Villa R Neurochem Res. 1998; 23(12):1485-91.

PMID: 9821151 DOI: 10.1023/a:1020907400905.

Changes of mitochondrial cytochrome c oxidase and FoF1 ATP synthase subunits in rat cerebral cortex during aging.

Nicoletti V, Tendi E, Lalicata C, Reale S, Costa A, Villa R Neurochem Res. 1995; 20(12):1465-70.

PMID: 8789609 DOI: 10.1007/BF00970595.

Effect of hypoxia on mitochondrial protein composition of cerebral cortex during aging.

Villa R, Turpeenoja L, Magri G, Gorini A, Ragusa N, Giuffrida-Stella A Neurochem Res. 1991; 16(7):821-6.

PMID: 1944772 DOI: 10.1007/BF00965692.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

VORBECK M, Martin A, Long Jr J, Smith J, Orr Jr R . Aging-dependent modification of lipid composition and lipid structural order parameter of hepatic mitochondria. Arch Biochem Biophys. 1982; 217(1):351-61. DOI: 10.1016/0003-9861(82)90511-2. View

Fando J, Salinas M, Wasterlain C . Age-dependent changes in brain protein synthesis in the rat. Neurochem Res. 1980; 5(4):373-83. DOI: 10.1007/BF00964226. View

Dwyer B, Fando J, Wasterlain C . Rat brain protein synthesis declines during postdevelopmental aging. J Neurochem. 1980; 35(3):746-9. DOI: 10.1111/j.1471-4159.1980.tb03717.x. View

De Robertis E, PELLEGRINO DE IRALDI A, RODRIGUEZ DE LORES GARNAIZ G, Salganicoff L . Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase. J Neurochem. 1962; 9:23-35. DOI: 10.1111/j.1471-4159.1962.tb07489.x. View

Cosgrove J, Atack J, Rapoport S . Regional analysis of rat brain proteins during senescence. Exp Gerontol. 1987; 22(3):187-98. DOI: 10.1016/0531-5565(87)90039-8. View

Starnes J, Edington D, Beyer R . Myocardial protein synthesis during aging and endurance exercise in rats. J Gerontol. 1983; 38(6):660-5. DOI: 10.1093/geronj/38.6.660. View

SANADI D . Age-related changes in cytochrome concentration of myocardial mitochondria. Mech Ageing Dev. 1978; 7(6):425-32. DOI: 10.1016/0047-6374(78)90083-0. View

Shahbazian F, Jacobs M, Lajtha A . Regional and cellular differences in rat brain protein synthesis in vivo and in slices during development. Int J Dev Neurosci. 1986; 4(3):209-15. DOI: 10.1016/0736-5748(86)90060-2. View

10.

Dunlop D, van Elden W, Lajtha A . Developmental effects on protein synthesis rates in regions of the CNS in vivo and in vitro. J Neurochem. 1977; 29(6):939-45. DOI: 10.1111/j.1471-4159.1977.tb06497.x. View

11.

Benzi G, Arrigoni E, Dagani F, Marzatico F, Curti D, Raimondo S . Age-dependent modification of drug interference on the enzymatic activities of the rat brain. Exp Gerontol. 1980; 15(6):593-603. DOI: 10.1016/0531-5565(80)90011-x. View

12.

Starnes J, Beyer R, Edington D . Myocardial adaptations to endurance exercise in aged rats. Am J Physiol. 1983; 245(4):H560-6. DOI: 10.1152/ajpheart.1983.245.4.H560. View

13.

Benzi G, GIUFFRIDA A . Changes of synaptosomal energy metabolism induced by hypoxia during aging. Neurochem Res. 1987; 12(2):149-57. DOI: 10.1007/BF00979531. View

14.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

15.

Turpeenoja L, Villa R, Magri G, Stella A . Changes of mitochondrial membrane proteins in rat cerebellum during aging. Neurochem Res. 1988; 13(9):859-65. DOI: 10.1007/BF00970754. View

16.

Avola R, Condorelli D, Ragusa N, Renis M, Alberghina M, Stella A . Protein synthesis rates in rat brain regions and subcellular fractions during aging. Neurochem Res. 1988; 13(4):337-42. DOI: 10.1007/BF00972483. View

17.

Hansford R . Bioenergetics in aging. Biochim Biophys Acta. 1983; 726(1):41-80. DOI: 10.1016/0304-4173(83)90010-1. View

18.

Gordon S, Finch C . An electrophoretic study of protein synthesis in brain regions of senescent male mice. Exp Gerontol. 1974; 9(5-6):267-73. DOI: 10.1016/0531-5565(74)90024-2. View

19.

Vitorica J, Clark A, Machado A, Satrustegui J . Impairment of glutamate uptake and absence of alterations in the energy-transducing ability of old rat brain mitochondria. Mech Ageing Dev. 1985; 29(3):255-66. DOI: 10.1016/0047-6374(85)90066-1. View