Cotranscription of the S10- and Spc-like Operons in Spinach Chloroplasts and Identification of Three of Their Gene Products

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1989 Apr 1

PMID 2747623

Citations 22

Authors

D X Zhou

F Quigley

O Massenet

R Mache

Affiliations

Soon will be listed here.

Abstract

The organisation and expression of the rpl22, rps3, rpl16 and rpl14 genes, which belong to the S10- and spc-like operons of spinach chloroplasts, have been studied. Northern experiments and nuclease S1 mapping show that the two operon-like groups of genes are cotranscribed. It is demonstrated that the intron-containing rpl16 gene is spliced in vivo. Based on amino acid composition and protein sequence data, the products of the rpl22, rpl16 and rpl14 genes are identified respectively as the spinach chloroplast ribosomal proteins CS-L13, CS-L24 and CS-L29. The rpl22 gene product is a 5S rRNA binding protein and therefore is distinguishable from the homologous Escherichia coli L22 ribosomal protein.

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