Presence in the Stroma of Chloroplasts of a Large Pool of a Ribosomal Protein Not Structurally Related to Any Escherichia Coli Ribosomal Protein

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1989 Oct 1

PMID 2693942

Citations 10

Authors

D X Zhou

R Mache

Affiliations

Soon will be listed here.

Abstract

A search was made for the presence of a pool of free ribosomal proteins in the stroma of the spinach chloroplast. The results showed that a relatively large amount of one protein, CS-S5, is present in the stroma. Immunoprecipitation experiments showed that this protein is encoded by the nuclear genome. Clones were isolated from a cDNA library constructed in the expression vector lambda gt11, using specific antibodies raised against the CS-S5 protein. A full-length cDNA was sequenced which contains an open reading frame (ORF) for the precursor of the CS-S5 protein, as shown by immunoprecipitation. This precursor contains a putative transit peptide of 66 amino acids and the mature product has no significant homology with any of the Escherichia coli ribosomal proteins, in contrast to the other ribosomal protein gene products so far identified in spinach chloroplasts.

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