Oncogenic Transformation of Drosophila Somatic Cells Induces a Functional PiRNA Pathway

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2016 Jul 31

PMID 27474441

Citations 23

Authors

Delphine Fagegaltier

Ilaria Falciatori

Benjamin Czech

Stephane Castel

Norbert Perrimon

Amanda Simcox

Gregory J Hannon

Affiliations

Soon will be listed here.

Abstract

Germline genes often become re-expressed in soma-derived human cancers as "cancer/testis antigens" (CTAs), and piRNA (PIWI-interacting RNA) pathway proteins are found among CTAs. However, whether and how the piRNA pathway contributes to oncogenesis in human neoplasms remain poorly understood. We found that oncogenic Ras combined with loss of the Hippo tumor suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from annotated generative loci, which are normally restricted to either the germline or the somatic follicle cells. Negating the pathway leads to increases in the expression of a wide variety of transposons and also altered expression of some protein-coding genes. This correlates with a reduction in the proliferation of the transformed cells in culture, suggesting that, at least in this context, the piRNA pathway may play a functional role in cancer.

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