Molecular Characterization and Expression Pattern of a Novel Keratin-associated Protein 11.1 Gene in the Liaoning Cashmere Goat ()

Overview

Journal Asian-Australas J Anim Sci

Specialty Veterinary Medicine

Date 2016 Jul 8

PMID 27383810

Citations 12

Authors

Mei Jin

Qian Cao

Ruilong Wang

Jun Piao

Fengqin Zhao

Jingai Piao

Affiliations

Soon will be listed here.

Abstract

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.

Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed . On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using hybridization.

Results: Bioinformatics analysis showed that gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, has a significantly higher expression in anagen than in catagen. Moreover, gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb.

Conclusion: We conclude that gene may play an important role in regulating the fiber diameter.

Citing Articles

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Multi-omics analysis of functional substances and expression verification in cashmere fineness.

Xu Y, Zhang Y, Qin Y, Gu M, Chen R, Sun Y BMC Genomics. 2023; 24(1):720.

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Identification of the Keratin-Associated Protein 22-2 Gene in the and Association of Its Variation with Cashmere Traits.

Chen Z, Cao J, Zhao F, He Z, Sun H, Wang J Animals (Basel). 2023; 13(17).

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Effects of exogenous melatonin on expressional differences of immune-related genes in cashmere goats.

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Integrated analysis of lncRNAs and mRNAs by RNA-Seq in secondary hair follicle development and cycling (anagen, catagen and telogen) of Jiangnan cashmere goat (Capra hircus).

Wu C, Qin C, Fu X, Huang X, Tian K BMC Vet Res. 2022; 18(1):167.

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