Molecular Characterization and Expression Pattern of a Novel Keratin-associated Protein 11.1 Gene in the Liaoning Cashmere Goat ()
Overview
Affiliations
Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.
Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed . On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using hybridization.
Results: Bioinformatics analysis showed that gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, has a significantly higher expression in anagen than in catagen. Moreover, gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb.
Conclusion: We conclude that gene may play an important role in regulating the fiber diameter.
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