Knocking Down of P53 Triggers Apoptosis and Autophagy, Concomitantly with Inhibition of Migration on SSC-4 Oral Squamous Carcinoma Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2016 Jul 3

PMID 27370646

Citations 16

Authors

Alexandra Iulia Irimie

Cornelia Braicu

Valentina Pileczki

Bobe Petrushev

Olga Soritau

Radu Septimiu Campian

Ioana Berindan-Neagoe

Affiliations

Soon will be listed here.

Abstract

Oral squamous cell carcinoma (OSCC) is a malignancy with elevated prevalence and somber prognosis due to the fact that most of the patients are diagnosed at an advanced stage. p53 has a crucial role in proliferation and apoptosis during the occurrence and development of numerous malignant tumors. The impact of mutated p53 on the development and progression of OSCC is unclear and might have therapeutic implications. Using an in vitro RNA interference experiment, we have evaluated the impact of p53 knockdown on cell viability, apoptosis, migration, and gene expression for key genes involved in apoptosis and angiogenesis. We observed that inhibiting the expression of p53 decreased the proliferation ability and induced apoptosis/autophagy in SSC-4 cells. Moreover, we observed that this has decreased migration and has blocked the expression of VEGF. In conclusion, our research provides a proof that a direct connection between p53 knockdown and OSCC cell death can be established, therefore opening new potential directions in OSCC molecular therapeutics and management.

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