Modulation of Mesenchymal Stem Cells with MiR-375 to Improve Their Therapeutic Outcome During Scar Formation

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2016 Jun 28

PMID 27347316

Citations 6

Authors

Wei Sheng

Zihao Feng

Qi Song

Heyong Niu

Guoying Miao

Affiliations

Soon will be listed here.

Abstract

Understanding of the mechanism of cutaneous scar formation with the goal of developing potential therapies to promote scar-less wound healing appears to be extremely critical. Mesenchymal stem cells (MSCs) have a demonstrate role in promoting scar-less wound healing. However, recent studies have shown that the function of MSCs may be attenuated due to insufficient activation in vivo. Here, we aimed to increase the activity and functions of MSCs to improve their effects during scar formation. We found that overexpression of microRNA-375 (miR-375) in MSCs significantly decreased the levels of tissue inhibitor of metalloproteinases 1 (TIMP-1) protein, but not mRNA. Mechanistically, miR-375 inhibited TIMP-1 protein translation through binding to the 3'-UTR of the TIMP-1 mRNA in MSCs. Transplantation of miR-375-expressing MSCs significantly reduced the fibrosis in the scar region of the mice, possibly through reduction of reactive oxygen species (ROS), suppression of transition of myofibroblasts from fibroblasts, and increases in hepatic growth factor (HGF). Together, these data suggest that overexpression of miR-375 in MSCs may substantially improve the effects of MSCs on reduction of scar during wound healing. Our study sheds new light on a scar-less wound healing.

Citing Articles

The Role of microRNA in the Inflammatory Response of Wound Healing.

Jiang Y, Xu X, Xiao L, Wang L, Qiang S Front Immunol. 2022; 13:852419.

PMID: 35386721 PMC: 8977525. DOI: 10.3389/fimmu.2022.852419.

Modulation of Mesenchymal Stem Cells for Enhanced Therapeutic Utility in Ischemic Vascular Diseases.

Elshaer S, Bahram S, Rajashekar P, Gangaraju R, El-Remessy A Int J Mol Sci. 2022; 23(1).

PMID: 35008675 PMC: 8745455. DOI: 10.3390/ijms23010249.

Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis.

Han B, Zhang Y, Xiao Y, Shi B, Wu H, Liu D Stem Cells Int. 2021; 2021:9964159.

PMID: 34257670 PMC: 8245228. DOI: 10.1155/2021/9964159.

MicroRNA‑375 prevents TGF‑β‑dependent transdifferentiation of lung fibroblasts via the MAP2K6/P38 pathway.

Zhang X, Chen Q, Song H, Jiang W, Xie S, Huang J Mol Med Rep. 2020; 22(3):1803-1810.

PMID: 32582987 PMC: 7411355. DOI: 10.3892/mmr.2020.11261.

Adeno-Associated Virus Vectors and Stem Cells: Friends or Foes?.

Brown N, Song L, Kollu N, Hirsch M Hum Gene Ther. 2017; 28(6):450-463.

PMID: 28490211 PMC: 5488260. DOI: 10.1089/hum.2017.038.

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