Bone Marrow-derived Mesenchymal Stem Cell Transplantation Ameliorates Oxidative Stress and Restores Intestinal Mucosal Permeability in Chemically Induced Colitis in Mice

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2015 Jul 16

PMID 26175850

Citations 26

Authors

Tao Sun

Guang-Zhou Gao

Rong-Fu Li

Xin Li

Da-Wei Li

Shan-Shan Wu

Anthony Et Yeo

Bo Jin

Affiliations

Soon will be listed here.

Abstract

Background: Ulcerative colitis (UC) can be viewed as an autoimmune disease. Bone marrow-derived mesenchymal stem cells (MSCs) with its regenerative, cellular multi-lineage and immunomodulatory abilities can influence the repair of damaged tissues in UC. This study investigated the effects of MSCs transplantation on the mice intestinal barrier in response to oxidative stress injury.

Methods: Colitis was induced by daily consecutive administration of 5% dextran sulfate sodium (DSS) solution for 7 days. Male murine MSCs were isolated and transplanted into female mice via injection in the tail vein. Serum and colon specimens were collected at 12 h, 24 h, 3 d, 7 d and 14 d after injection. Serum levels of D-lactate (D-LAC), diamine oxidase (DAO), colonic levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified. The SRY protein of the male sex determinant gene expression and E-cadherin were also ascertained intracellularly.

Results: Three days after receiving male MSCs transplantation, SRY protein expression was detected. The quantity increased on successive days. Serum levels of D-LAC and DAO, colonic MDA and SOD normalized in a shorter time period compared to controls (p<0.05). Not surprisingly, histological regeneration of tissue and E-cadherin expression in the colon of MSCs transplanted mice also occurred in a shorter time period than controls.

Conclusions: Transplanted MSCs restored mucosal permeability, and minimized oxidative stress related injury.

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References

Randhawa P, Singh K, Singh N, Jaggi A . A review on chemical-induced inflammatory bowel disease models in rodents. Korean J Physiol Pharmacol. 2014; 18(4):279-88. PMC: 4146629. DOI: 10.4196/kjpp.2014.18.4.279. View

Zhang J, Gong J, Zhang W, Zhu W, Li J . Effects of transplanted bone marrow mesenchymal stem cells on the irradiated intestine of mice. J Biomed Sci. 2008; 15(5):585-94. DOI: 10.1007/s11373-008-9256-9. View

Murray M, Barbose J, Cobb C . Serum D(-)-lactate levels as a predictor of acute intestinal ischemia in a rat model. J Surg Res. 1993; 54(5):507-9. DOI: 10.1006/jsre.1993.1078. View

Komori M, Tsuji S, Tsujii M, Murata H, Iijima H, Yasumaru M . Involvement of bone marrow-derived cells in healing of experimental colitis in rats. Wound Repair Regen. 2005; 13(1):109-18. DOI: 10.1111/j.1067-1927.2005.130114.x. View

Murano M, Maemura K, Hirata I, Toshina K, Nishikawa T, Hamamoto N . Therapeutic effect of intracolonically administered nuclear factor kappa B (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis. Clin Exp Immunol. 2000; 120(1):51-8. PMC: 1905625. DOI: 10.1046/j.1365-2249.2000.01183.x. View

Del Rio D, Stewart A, Pellegrini N . A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005; 15(4):316-28. DOI: 10.1016/j.numecd.2005.05.003. View

Rosenberg L, Peppercorn M . Efficacy and safety of drugs for ulcerative colitis. Expert Opin Drug Saf. 2010; 9(4):573-92. DOI: 10.1517/14740331003639412. View

Dogan A, Wang Z, Spencer J . E-cadherin expression in intestinal epithelium. J Clin Pathol. 1995; 48(2):143-6. PMC: 502385. DOI: 10.1136/jcp.48.2.143. View

Jena G, Trivedi P, Sandala B . Oxidative stress in ulcerative colitis: an old concept but a new concern. Free Radic Res. 2012; 46(11):1339-45. DOI: 10.3109/10715762.2012.717692. View

10.

He X, He X, Lian L, Wu X, Lan P . Systemic infusion of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis in mice. Dig Dis Sci. 2012; 57(12):3136-44. DOI: 10.1007/s10620-012-2290-5. View

11.

Song C, Li G . CXCR4 and matrix metalloproteinase-2 are involved in mesenchymal stromal cell homing and engraftment to tumors. Cytotherapy. 2010; 13(5):549-61. DOI: 10.3109/14653249.2010.542457. View

12.

Jackson W, Nesti L, Tuan R . Concise review: clinical translation of wound healing therapies based on mesenchymal stem cells. Stem Cells Transl Med. 2012; 1(1):44-50. PMC: 3727688. DOI: 10.5966/sctm.2011-0024. View

13.

Khalil P, Weiler V, Nelson P, Khalil M, Moosmann S, Mutschler W . Nonmyeloablative stem cell therapy enhances microcirculation and tissue regeneration in murine inflammatory bowel disease. Gastroenterology. 2007; 132(3):944-54. DOI: 10.1053/j.gastro.2006.12.029. View

14.

Jiang H, Qu L, Li Y, Gu L, Shi Y, Zhang J . Bone marrow mesenchymal stem cells reduce intestinal ischemia/reperfusion injuries in rats. J Surg Res. 2009; 168(1):127-34. DOI: 10.1016/j.jss.2009.07.035. View

15.

Chen X, McClurg A, Zhou G, McCaigue M, Armstrong M, Li G . Chondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules. Stem Cells. 2006; 25(2):364-70. DOI: 10.1634/stemcells.2006-0268. View

16.

Ahuja V, Tandon R . Inflammatory bowel disease in the Asia-Pacific area: a comparison with developed countries and regional differences. J Dig Dis. 2010; 11(3):134-47. DOI: 10.1111/j.1751-2980.2010.00429.x. View

17.

Danese S, Fiocchi C . Ulcerative colitis. N Engl J Med. 2011; 365(18):1713-25. DOI: 10.1056/NEJMra1102942. View

18.

Scaldaferri F, Pizzoferrato M, Gerardi V, Lopetuso L, Gasbarrini A . The gut barrier: new acquisitions and therapeutic approaches. J Clin Gastroenterol. 2012; 46 Suppl:S12-7. DOI: 10.1097/MCG.0b013e31826ae849. View

19.

Hermiston M, Wong M, Gordon J . Forced expression of E-cadherin in the mouse intestinal epithelium slows cell migration and provides evidence for nonautonomous regulation of cell fate in a self-renewing system. Genes Dev. 1996; 10(8):985-96. DOI: 10.1101/gad.10.8.985. View

20.

Hayashi Y, Tsuji S, Tsujii M, Nishida T, Ishii S, Iijima H . Topical implantation of mesenchymal stem cells has beneficial effects on healing of experimental colitis in rats. J Pharmacol Exp Ther. 2008; 326(2):523-31. DOI: 10.1124/jpet.108.137083. View