Changes in the Chemical Barrier Composition of Tears in Alzheimer's Disease Reveal Potential Tear Diagnostic Biomarkers

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Jun 22

PMID 27327445

Citations 59

Authors

Gergo Kallo

Miklos Emri

Zsofia Varga

Bernadett Ujhelyi

Jozsef Tozser

Adrienne Csutak

Eva Csosz

Affiliations

Soon will be listed here.

Abstract

Alzheimer's disease (AD) is one of the most common neurodegenerative diseases, with increasing prevalence affecting millions of people worldwide. Currently, only autopsy is able to confirm the diagnosis with a 100% certainty, therefore, biomarkers from body fluids obtained by non-invasive means provide an attractive alternative for the diagnosis of Alzheimer`s disease. Global changes of the protein profile were examined by quantitative proteomics; firstly, electrophoresis and LC-MS/MS were used, thereafter, SRM-based targeted proteomics method was developed and applied to examine quantitative changes of tear proteins. Alterations in the tear flow rate, total tear protein concentration and composition of the chemical barrier specific to AD were demonstrated, and the combination of lipocalin-1, dermcidin, lysozyme-C and lacritin was shown to be a potential biomarker, with an 81% sensitivity and 77% specificity.

Citing Articles

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High-Throughput Tear Proteomics via In-Capillary Digestion for Biomarker Discovery.

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