Typing of Clostridium Difficile Causing Diarrhoea in an Orthopaedic Ward

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1989 May 1

PMID 2732346

Citations 7

Authors

I McKAY

J E Coia

I R Poxton

Affiliations

Soon will be listed here.

Abstract

In an outbreak of diarrhoeal disease in an orthopaedic ward Clostridium difficile was isolated from all six patients with diarrhoea. Attempts were made to type these isolates by means of antibiogram, detection of pre-formed enzymes, analysis of surface proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting, and plasmid profile analysis. This showed that a single strain (type E) indistinguishable by the four distinct methods of typing, was isolated from all six patients at some time during their episodes of diarrhoea. Relapse was caused by the acquisition of a new strain in two patients, and by re-emergence or reacquisition of the original strain in two patients. The immunochemical method was the most sensitive and discriminatory of the typing strategies adopted.

Citing Articles

Survey of incidence of Clostridium difficile infection in Canadian hospitals and diagnostic approaches.

Alfa M, Du T, Beda G J Clin Microbiol. 1998; 36(7):2076-80.

PMID: 9650966 PMC: 104982. DOI: 10.1128/JCM.36.7.2076-2080.1998.

Investigation of an outbreak of Clostridium difficile infection in a general hospital by numerical analysis of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Costas M, Holmes B, On S, Ganner M, Kelly M, Nath S J Clin Microbiol. 1994; 32(3):759-65.

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Comparison of typing methods for Clostridium difficile isolates.

Wolfhagen M, Fluit A, Torensma R, Jansze M, Kuypers A, Verhage E J Clin Microbiol. 1993; 31(8):2208-11.

PMID: 8103774 PMC: 265725. DOI: 10.1128/jcm.31.8.2208-2211.1993.

Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.

Costas M, Holmes B, Ganner M, On S, Hoffman P, Worsley M Epidemiol Infect. 1994; 113(1):1-12.

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Rapid detection of toxigenic Clostridium difficile in fecal samples by magnetic immuno PCR assay.

Wolfhagen M, Fluit A, Torensma R, Poppelier M, Verhoef J J Clin Microbiol. 1994; 32(7):1629-33.

PMID: 7929748 PMC: 263744. DOI: 10.1128/jcm.32.7.1629-1633.1994.

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