Evaluation of the XTAG Gastrointestinal Pathogen Panel Assay for the Detection of Enteric Pathogens in Kuwait

Overview

Journal Med Princ Pract

Publisher Karger

Specialties General Medicine
Medical Education

Date 2016 Jun 21

PMID 27322647

Citations 10

Authors

Manuel John Albert

Vincent Olubunmi Rotimi

Jamshed Iqbal

Wassim Chehadeh

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the utility of the Luminex xTAG gastrointestinal pathogen panel (GPP) assay in the detection of enteric pathogens from diarrheal stool samples in Kuwait.

Materials And Methods: The Luminex xTAG GPP assay was used according to the manufacturer's instructions to evaluate single diarrheal stool samples from 109 hospitalized patients at Mubarak Al-Kabeer Hospital, Kuwait, from March 2014 to June 2015. The assay procedure involved nucleic acid extraction from stool samples, amplification of the target by reverse transcriptase polymerase chain reaction, hybridization of the amplified target by probe, detection of the target by the Luminex instrument and computerized data analysis. Conventional microbiological assays were used as the gold standard for comparison.

Results: From the 109 diarrheal stool samples, 20 (18.4%) pathogens were detected by the xTAG GPP assay compared to 10 (9.2%) pathogens using conventional assays. Both methods detected 3 Salmonella spp., 3 Clostridium difficile, 2 rotavirus and 2 norovirus. In addition, the xTAG GPP assay detected 1 Shigella sp., 6 Campylobacter spp., 1 Cryptosporidium sp. and 2 Giardia lamblia which were missed by conventional assays.

Conclusions: In this study, xTAG GPP detected twice as many pathogens as the conventional assays. We recommend the introduction of this assay in routine diagnostic laboratories for a rapid and better diagnosis and treatment of diarrheal disease.

Citing Articles

Comparative Evaluation of Luminex xTAG Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of Toxin in Stool Samples from a Pediatric Population.

Tyrrell H, Morin S, Lloyd C, Parsons B, Stokowski T, Xie J Microorganisms. 2022; 10(11).

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and Cryptosporidiosis: The Perspective from the Gulf Countries.

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Burden of Infection among Patients in Western Asia: A Systematic Review and Meta-Analysis.

Malekzadegan Y, Halaji M, Hasannejad-Bibalan M, Jalalifar S, Fathi J, Ebrahim-Saraie H Iran J Public Health. 2019; 48(9):1589-1599.

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In vitro spleen cell cytokine responses of adult mice immunized with a recombinant PorA (major outer membrane protein [MOMP]) from Campylobacter jejuni.

Albert M, Raghupathy R, Khan I, Azizieh F Sci Rep. 2019; 9(1):12024.

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Non-typhoidal Salmonella blood stream infection in Kuwait: Clinical and microbiological characteristics.

Albert M, Bulach D, Alfouzan W, Izumiya H, Carter G, Alobaid K PLoS Negl Trop Dis. 2019; 13(4):e0007293.

PMID: 30986214 PMC: 6483562. DOI: 10.1371/journal.pntd.0007293.

References

Beckmann C, Heininger U, Marti H, Hirsch H . Gastrointestinal pathogens detected by multiplex nucleic acid amplification testing in stools of pediatric patients and patients returning from the tropics. Infection. 2014; 42(6):961-70. DOI: 10.1007/s15010-014-0656-7. View

Zboromyrska Y, Hurtado J, Salvador P, Alvarez-Martinez M, Valls M, Mas J . Aetiology of traveller's diarrhoea: evaluation of a multiplex PCR tool to detect different enteropathogens. Clin Microbiol Infect. 2014; 20(10):O753-9. DOI: 10.1111/1469-0691.12621. View

Xu W, McDonough M, Erdman D . Species-specific identification of human adenoviruses by a multiplex PCR assay. J Clin Microbiol. 2000; 38(11):4114-20. PMC: 87550. DOI: 10.1128/JCM.38.11.4114-4120.2000. View

Johnston S, Ballard M, Beach M, Causer L, Wilkins P . Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens. J Clin Microbiol. 2003; 41(2):623-6. PMC: 149727. DOI: 10.1128/JCM.41.2.623-626.2003. View

Al-Rashidi A, Chehadeh W, Szucs G, Albert M . Different norovirus genotypes in patients with gastroenteritis in Kuwait. J Med Virol. 2013; 85(9):1611-8. DOI: 10.1002/jmv.23645. View

Goldenberg S, Bacelar M, Brazier P, Bisnauthsing K, Edgeworth J . A cost benefit analysis of the Luminex xTAG Gastrointestinal Pathogen Panel for detection of infectious gastroenteritis in hospitalised patients. J Infect. 2014; 70(5):504-11. DOI: 10.1016/j.jinf.2014.11.009. View

Mengelle C, Mansuy J, Prere M, Grouteau E, Claudet I, Kamar N . Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients. Clin Microbiol Infect. 2013; 19(10):E458-65. DOI: 10.1111/1469-0691.12255. View

Albert M, Rotimi V, Dhar R, Silpikurian S, Pacsa A, Molla A . Diarrhoeagenic Escherichia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait. BMC Microbiol. 2009; 9:62. PMC: 2678128. DOI: 10.1186/1471-2180-9-62. View

Voetsch A, Angulo F, Rabatsky-Ehr T, Shallow S, Cassidy M, Thomas S . Laboratory practices for stool-specimen culture for bacterial pathogens, including Escherichia coli O157:H7, in the FoodNet sites, 1995-2000. Clin Infect Dis. 2004; 38 Suppl 3:S190-7. DOI: 10.1086/381586. View

10.

Iqbal J, Hira P, Al-Ali F, Philip R . Cryptosporidiosis in Kuwaiti children: seasonality and endemicity. Clin Microbiol Infect. 2001; 7(5):261-6. DOI: 10.1046/j.1198-743x.2001.00254.x. View

11.

de Boer R, Ott A, Kesztyus B, Kooistra-Smid A . Improved detection of five major gastrointestinal pathogens by use of a molecular screening approach. J Clin Microbiol. 2010; 48(11):4140-6. PMC: 3020836. DOI: 10.1128/JCM.01124-10. View

12.

Platts-Mills J, Liu J, Houpt E . New concepts in diagnostics for infectious diarrhea. Mucosal Immunol. 2013; 6(5):876-85. DOI: 10.1038/mi.2013.50. View

13.

Navidad J, Griswold D, Gradus M, Bhattacharyya S . Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. J Clin Microbiol. 2013; 51(9):3018-24. PMC: 3754681. DOI: 10.1128/JCM.00896-13. View

14.

Claas E, Burnham C, Mazzulli T, Templeton K, Topin F . Performance of the xTAG® gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis. J Microbiol Biotechnol. 2013; 23(7):1041-5. DOI: 10.4014/jmb.1212.12042. View

15.

Albert M, Faruque A, Faruque S, Sack R, Mahalanabis D . Case-control study of enteropathogens associated with childhood diarrhea in Dhaka, Bangladesh. J Clin Microbiol. 1999; 37(11):3458-64. PMC: 85667. DOI: 10.1128/JCM.37.11.3458-3464.1999. View

16.

Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J . PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia. J Clin Microbiol. 2007; 45(3):1035-7. PMC: 1829108. DOI: 10.1128/JCM.02144-06. View

17.

Valcin C, Severe K, Riche C, Anglade B, Moise C, Woodworth M . Predictors of disease severity in patients admitted to a cholera treatment center in urban Haiti. Am J Trop Med Hyg. 2013; 89(4):625-632. PMC: 3795091. DOI: 10.4269/ajtmh.13-0170. View

18.

Sethi S, Khuffash F, Al-Nakib W . Microbial etiology of acute gastroenteritis in hospitalized children in Kuwait. Pediatr Infect Dis J. 1989; 8(9):593-7. DOI: 10.1097/00006454-198909000-00005. View

19.

Perry M, Corden S, Howe R . Evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel and the Savyon Diagnostics Gastrointestinal Infection Panel for the detection of enteric pathogens in clinical samples. J Med Microbiol. 2014; 63(Pt 11):1419-1426. DOI: 10.1099/jmm.0.074773-0. View

20.

Malecki M, Schildgen V, Kamm M, Mattner F, Schildgen O . Rapid screening method for multiple gastroenteric pathogens also detects novel enterohemorrhagic Escherichia coli O104:H4. Am J Infect Control. 2011; 40(1):82-3. DOI: 10.1016/j.ajic.2011.07.019. View