Single-cell Analysis: Advances and Future Perspectives

Overview

Journal Bosn J Basic Med Sci

Specialty General Medicine

Date 2016 Jun 21

PMID 27320288

Citations 13

Authors

Emir Hodzic

Affiliations

Soon will be listed here.

Abstract

The last several years have seen rapid development of technologies and methods that permit a detailed analysis of the genome and transcriptome of a single cell. Recent evidence from studies of single cells reveals that each cell type has a distinct lineage and function. The lineage and stage of development of each cell determine how they respond to each other and the environment. Experimental approaches that utilize single-cell analysis are effective means to understand how cell networks work in concert to coordinate a response at the population level; recent progress in single-cell analysis is offering a glimpse at the future.

Citing Articles

Dielectrophoretic capture and electrochemical enzyme-linked immunosorbent assay of single melanoma cells at an array of interlocked spiral bipolar electrodes.

Clark M, Moser H, Anand R ChemElectroChem. 2024; 11(15).

PMID: 39483376 PMC: 11526340. DOI: 10.1002/celc.202400182.

Stochastic journeys of cell progenies through compartments and the role of self-renewal, symmetric and asymmetric division.

Dreiwi H, Feliciangeli F, Castro M, Lythe G, Molina-Paris C, Lopez-Garcia M Sci Rep. 2024; 14(1):16287.

PMID: 39009631 PMC: 11251179. DOI: 10.1038/s41598-024-63500-2.

Optical diffraction tomography for assessing single cell models in angular light scattering.

Dunn K, Matlock A, Funkenbusch G, Yaqoob Z, So P, Berger A Biomed Opt Express. 2024; 15(2):973-990.

PMID: 38404316 PMC: 10890861. DOI: 10.1364/BOE.512149.

Digital PCR for Single-Cell Analysis.

Fang W, Liu X, Maiga M, Cao W, Mu Y, Yan Q Biosensors (Basel). 2024; 14(2).

PMID: 38391982 PMC: 10886679. DOI: 10.3390/bios14020064.

Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists.

van Doorn E, Amesz J, Sadeghi A, de Groot N, Manintveld O, Taverne Y Cardiovasc Eng Technol. 2024; 15(2):232-249.

PMID: 38228811 PMC: 11116217. DOI: 10.1007/s13239-023-00707-w.

References

Navin N, Hicks J . Future medical applications of single-cell sequencing in cancer. Genome Med. 2011; 3(5):31. PMC: 3219072. DOI: 10.1186/gm247. View

Heath J, Ribas A, Mischel P . Single-cell analysis tools for drug discovery and development. Nat Rev Drug Discov. 2015; 15(3):204-16. PMC: 4883669. DOI: 10.1038/nrd.2015.16. View

Tang F, Lao K, Surani M . Development and applications of single-cell transcriptome analysis. Nat Methods. 2011; 8(4 Suppl):S6-11. PMC: 3408593. DOI: 10.1038/nmeth.1557. View

Navin N, Kendall J, Troge J, Andrews P, Rodgers L, McIndoo J . Tumour evolution inferred by single-cell sequencing. Nature. 2011; 472(7341):90-4. PMC: 4504184. DOI: 10.1038/nature09807. View

Shapiro E, Biezuner T, Linnarsson S . Single-cell sequencing-based technologies will revolutionize whole-organism science. Nat Rev Genet. 2013; 14(9):618-30. DOI: 10.1038/nrg3542. View

Albayrak C, Jordi C, Zechner C, Lin J, Bichsel C, Khammash M . Digital Quantification of Proteins and mRNA in Single Mammalian Cells. Mol Cell. 2016; 61(6):914-24. DOI: 10.1016/j.molcel.2016.02.030. View

Saliba A, Westermann A, Gorski S, Vogel J . Single-cell RNA-seq: advances and future challenges. Nucleic Acids Res. 2014; 42(14):8845-60. PMC: 4132710. DOI: 10.1093/nar/gku555. View

Stahlberg A, Thomsen C, Ruff D, Aman P . Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell. Clin Chem. 2012; 58(12):1682-91. DOI: 10.1373/clinchem.2012.191445. View

Yoshimoto N, Kida A, Jie X, Kurokawa M, Iijima M, Niimi T . An automated system for high-throughput single cell-based breeding. Sci Rep. 2013; 3:1191. PMC: 3561619. DOI: 10.1038/srep01191. View

10.

Macaulay I, Voet T . Single cell genomics: advances and future perspectives. PLoS Genet. 2014; 10(1):e1004126. PMC: 3907301. DOI: 10.1371/journal.pgen.1004126. View

11.

Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R . Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011; 332(6030):687-96. PMC: 3273988. DOI: 10.1126/science.1198704. View

12.

Rubakhin S, Romanova E, Nemes P, Sweedler J . Profiling metabolites and peptides in single cells. Nat Methods. 2011; 8(4 Suppl):S20-9. PMC: 3312877. DOI: 10.1038/nmeth.1549. View

13.

Shalek A, Satija R, Adiconis X, Gertner R, Gaublomme J, Raychowdhury R . Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells. Nature. 2013; 498(7453):236-40. PMC: 3683364. DOI: 10.1038/nature12172. View

14.

Lovatt D, Ruble B, Lee J, Dueck H, Kim T, Fisher S . Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue. Nat Methods. 2014; 11(2):190-6. PMC: 3964595. DOI: 10.1038/nmeth.2804. View

15.

Yilmaz S, Singh A . Single cell genome sequencing. Curr Opin Biotechnol. 2011; 23(3):437-43. PMC: 3318999. DOI: 10.1016/j.copbio.2011.11.018. View

16.

Georgiou G, Ippolito G, Beausang J, Busse C, Wardemann H, Quake S . The promise and challenge of high-throughput sequencing of the antibody repertoire. Nat Biotechnol. 2014; 32(2):158-68. PMC: 4113560. DOI: 10.1038/nbt.2782. View