Inhibitory Effects of Willd. on Colorectal Cancer Stem Cells

Overview

Journal Oncol Lett

Specialty Oncology

Date 2016 Jun 18

PMID 27313710

Citations 22

Authors

Guodong Sun

Lihui Wei

Jianyu Feng

Jiumao Lin

Jun Peng

Affiliations

Soon will be listed here.

Abstract

Cancer stem cells (CSCs) are proposed to be closely correlated with the development and progression of tumors, as well as with chemo- and radioresistance. Targeting CSCs may therefore be a promising potential strategy for the treatment of cancer. Currently, natural products have received great interest due to their therapeutic efficacy and reduced adverse effects compared with modern chemotherapeutics. As a significant component of a number of traditional Chinese medicine formulas, the medicinal herb Willd. (HDW) has long been utilized in China to clinically treat a variety of malignancies, including colorectal cancer (CRC). Previously, the authors of the present study reported that HDW suppressed CRC growth through multiple mechanisms, including promoting apoptosis, and inhibiting cell proliferation and tumor angiogenesis. To additionally investigate its mode of action, the present study isolated a stem-like side population (SP) from colorectal cancer HT-29 cells to investigate the effect of ethanol extract of HDW on CSCs. It was observed that HDW was able to markedly downregulate the expression of CSC marker leucine-rich repeat-containing G-protein coupled receptor 5 and also significantly decrease the proportion of SP in HT-29 cells, in a dose-dependent manner. Furthermore, HDW treatment significantly and dose-dependently inhibited the viability and sphere formation, and induced cell morphological changes of isolated HT-29 SP cells. In addition, HDW greatly suppressed the messenger RNA expression of several critical genes that mediate CSC features, including ATP-binding cassette, sub-family B, member 1, β-catenin, c-Myc, proliferating cell nuclear antigen and survivin. In conclusion, the present study indicates that HDW may exert inhibitory effects on cancer stem cells.

Citing Articles

Molecular Targets and Mechanisms of Hedyotis diffusa Willd. for Esophageal Adenocarcinoma Treatment Based on Network Pharmacology and Weighted Gene Co-expression Network Analysis.

Zhuang Y, Sun Y, Wang C, Zhang Q, Che C, Shao F Curr Drug Targets. 2024; 25(6):431-443.

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Phytochemistry, pharmacology, and medical uses of Oldenlandia (family Rubaceae): a review.

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Molecular Mechanism of Anti-Colorectal Cancer Effect of Willd and Its Extracts.

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Adjunctive Chinese Herbal Medicine Treatment is Associated With an Improved Survival Rate in Patients With Cervical Cancer in Taiwan: A Matched Cohort Study.

Wang C, Lin K, Wu M, Lin C, Lin J, Chang C Integr Cancer Ther. 2021; 20:15347354211061752.

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