Rapid, Culture-Free Detection of Staphylococcus Aureus Bacteremia

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Jun 16

PMID 27305148

Citations 6

Authors

Elliot L Burghardt

Katie S Flenker

Karen C Clark

Jeff Miguel

Dilek Ince

Patricia Winokur

Bradley Ford

James O McNamara 2nd

Affiliations

Soon will be listed here.

Abstract

S. aureus bacteremia (SAB) is a common condition with high rates of morbidity and mortality. Current methods used to diagnose SAB take at least a day, and often longer. Patients with suspected bacteremia must therefore be empirically treated, often unnecessarily, while assay results are pending. In this proof-of-concept study, we describe an inexpensive assay that detects SAB via the detection of micrococcal nuclease (an enzyme secreted by S. aureus) in patient plasma samples in less than three hours. In total, 17 patient plasma samples from culture-confirmed S. aureus bacteremic individuals were tested. 16 of these yielded greater nuclease assay signals than samples from uninfected controls or individuals with non-S. aureus bacteremia. These results suggest that a nuclease-detecting assay may enable the rapid and inexpensive diagnosis of SAB, which is expected to substantially reduce the mortality and morbidity that result from this condition.

Citing Articles

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Ex Vivo Tracer Efficacy in Optical Imaging of Staphylococcus Aureus Nuclease Activity.

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