Binding Equilibrium and Kinetics of Membrane-anchored Receptors and Ligands in Cell Adhesion: Insights from Computational Model Systems and Theory

Overview

Journal Cell Adh Migr

Specialty Cell Biology

Date 2016 Jun 14

PMID 27294442

Citations 10

Authors

Thomas R Weikl

Jinglei Hu

Guang-Kui Xu

Reinhard Lipowsky

Affiliations

Soon will be listed here.

Abstract

The adhesion of cell membranes is mediated by the binding of membrane-anchored receptor and ligand proteins. In this article, we review recent results from simulations and theory that lead to novel insights on how the binding equilibrium and kinetics of these proteins is affected by the membranes and by the membrane anchoring and molecular properties of the proteins. Simulations and theory both indicate that the binding equilibrium constant [Formula: see text] and the on- and off-rate constants of anchored receptors and ligands in their 2-dimensional (2D) membrane environment strongly depend on the membrane roughness from thermally excited shape fluctuations on nanoscales. Recent theory corroborated by simulations provides a general relation between [Formula: see text] and the binding constant [Formula: see text] of soluble variants of the receptors and ligands that lack the membrane anchors and are free to diffuse in 3 dimensions (3D).

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