Neurokinin B-related Peptide Suppresses the Expression of GnRH I, Kiss2 and Tac3 in the Brain of Mature Female Nile Tilapia Oreochromis Niloticus

Overview

Journal Dev Reprod

Specialty Biology

Date 2016 Jun 14

PMID 27294210

Citations 5

Authors

Ye Hwa Jin

Jin Woo Park

Jung-Hyun Kim

Joon Yeong Kwon

Affiliations

Soon will be listed here.

Abstract

Neurokinin B (NKB) and neurokinin B related peptide (NKBRP) belong to tachykinin peptide family. Theyact as a neurotransmitter and/or neuromodulator. Mutation of NKB and/or its cognate receptor, NK3R resulted in hypogonadotropic hypogonadism in mammals, implying a strong involvement of NKB/NK3R system in controlling mammalian reproduction. Teleosts possess NKBRP as well as NKB, but their roles in fish reproduction need to be clarified. In this study, NKB and NKBRP coding gene (tac3) was cloned from Nile tilapia and sequenced. Based on the sequence, Nile tilapia NKB and NKBRP peptide were synthesized and their biological potencies were tested in vitro pituitary culture. The synthetic NKBRP showed direct inhibitory effect on the expression of GTH subunits at the pituitary level. This inhibitory effect was confirmed in vivo by means of intraperitoneal (ip) injection of synthetic NKB and NKBRP to mature female tilapia (20 pmol/g body weight [BW]). Both NKB and NKBRP had no effect on the plasma level of sex steroids, E2 and 11-KT. However, NKBRP caused declines of expression level of GnRH I, Kiss2 and tac3 mRNAs in the brain while NKB seemed to have no distinct effect. These results indicate some inhibitory roles of NKBRP in reproduction of mature female Nile tilapia, although their exact functions are not clear at the moment.

Citing Articles

Tachykinins, new players in the control of reproduction and food intake: A comparative review in mammals and teleosts.

Campo A, Dufour S, Rousseau K Front Endocrinol (Lausanne). 2023; 13:1056939.

PMID: 36589829 PMC: 9800884. DOI: 10.3389/fendo.2022.1056939.

Response of Pituitary Cells and Tissues to Neurokinin B and F in the Nile tilapia.

Mun S, Oh H, Kwon J Dev Reprod. 2022; 26(1):13-21.

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Effects of Kiss2 on the Expression of Gonadotropin Genes in the Pituitary of Nile Tilapia ().

Park J, Kim J, Kwon J Dev Reprod. 2020; 24(3):149-158.

PMID: 33110946 PMC: 7576967. DOI: 10.12717/DR.2020.24.3.149.

Tachykinin-3 Genes and Peptides Characterized in a Basal Teleost, the European Eel: Evolutionary Perspective and Pituitary Role.

Campo A, Lafont A, Lefranc B, Leprince J, Tostivint H, Kamech N Front Endocrinol (Lausanne). 2018; 9:304.

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