Three Rat Preprotachykinin MRNAs Encode the Neuropeptides Substance P and Neurokinin A

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Feb 1

PMID 2433692

Citations 71

Authors

J E Krause

J M Chirgwin

M S Carter

Z S Xu

A D HERSHEY

Affiliations

Soon will be listed here.

Abstract

Synthetic oligonucleotides were used to screen a rat striatal cDNA library for sequences corresponding to the tachykinin peptides substance P and neurokinin A. The cDNA library was constructed from RNA isolated from the rostral portion of the rat corpus striatum, the site of striatonigral cell bodies. Two types of cDNAs were isolated and defined by restriction enzyme analysis and DNA sequencing to encode both substance P and neurokinin A. The two predicted preprotachykinin protein precursors (130 and 115 amino acids in length) differ from each other by a pentadecapeptide sequence between the two tachykinin sequences, and both precursors possess appropriate processing signals for substance P and neurokinin A production. The presence of a third preprotachykinin mRNA of minor abundance in rat striatum was established by S1 nuclease protection experiments. This mRNA encodes a preprotachykinin of 112 amino acids containing substance P but not neurokinin A. These three mRNAs are derived from one rat gene as a result of differential RNA processing; thus, this RNA processing pattern further increases the diversity of products that can be generated from the preprotachykinin gene.

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References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Lehrach H, Diamond D, Wozney J, Boedtker H . RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977; 16(21):4743-51. DOI: 10.1021/bi00640a033. View

Smyth D, Massey D . A new glycopeptide in pig, ox and sheep pituitary. Biochem Biophys Res Commun. 1979; 87(4):1006-10. DOI: 10.1016/s0006-291x(79)80007-8. View

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

Favaloro J, Treisman R, Kamen R . Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980; 65(1):718-49. DOI: 10.1016/s0076-6879(80)65070-8. View

Nicoll R, Schenker C, Leeman S . Substance P as a transmitter candidate. Annu Rev Neurosci. 1980; 3:227-68. DOI: 10.1146/annurev.ne.03.030180.001303. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Peacock S, McIver C, Monahan J . Transformation of E. coli using homopolymer-linked plasmid chimeras. Biochim Biophys Acta. 1981; 655(2):243-50. DOI: 10.1016/0005-2787(81)90014-9. View

Bradbury A, Finnie M, Smyth D . Mechanism of C-terminal amide formation by pituitary enzymes. Nature. 1982; 298(5875):686-8. DOI: 10.1038/298686a0. View

10.

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

11.

Biggin M, Gibson T, Hong G . Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983; 80(13):3963-5. PMC: 394179. DOI: 10.1073/pnas.80.13.3963. View

12.

Nevins J . The pathway of eukaryotic mRNA formation. Annu Rev Biochem. 1983; 52:441-66. DOI: 10.1146/annurev.bi.52.070183.002301. View

13.

Nawa H, Hirose T, Takashima H, INAYAMA S, Nakanishi S . Nucleotide sequences of cloned cDNAs for two types of bovine brain substance P precursor. Nature. 1983; 306(5938):32-6. DOI: 10.1038/306032a0. View

14.

Gubler U, HOFFMAN B . A simple and very efficient method for generating cDNA libraries. Gene. 1983; 25(2-3):263-9. DOI: 10.1016/0378-1119(83)90230-5. View

15.

Queen C, Korn L . A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 1984; 12(1 Pt 2):581-99. PMC: 321072. DOI: 10.1093/nar/12.1part2.581. View

16.

Krause J, Reiner A, Advis J, McKelvy J . In vivo biosynthesis of [35S]- and [3H]substance P in the striatum of the rat and their axonal transport to the substantia nigra. J Neurosci. 1984; 4(3):775-85. PMC: 6564840. View

17.

von Heijne G . How signal sequences maintain cleavage specificity. J Mol Biol. 1984; 173(2):243-51. DOI: 10.1016/0022-2836(84)90192-x. View

18.

Kozak M . Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin. Nucleic Acids Res. 1984; 12(9):3873-93. PMC: 318796. DOI: 10.1093/nar/12.9.3873. View

19.

Itakura K, Rossi J, Wallace R . Synthesis and use of synthetic oligonucleotides. Annu Rev Biochem. 1984; 53:323-56. DOI: 10.1146/annurev.bi.53.070184.001543. View

20.

Douglass J, Civelli O, Herbert E . Polyprotein gene expression: generation of diversity of neuroendocrine peptides. Annu Rev Biochem. 1984; 53:665-715. DOI: 10.1146/annurev.bi.53.070184.003313. View