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On Methylene-bridged Cysteine and Lysine Residues in Proteins

Overview
Journal Protein Sci
Specialty Biochemistry
Date 2016 Jun 5
PMID 27261771
Citations 8
Authors
Milosz Ruszkowski
Zbigniew Dauter
Affiliations
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Abstract

Cysteine residues ubiquitously stabilize tertiary and quaternary protein structure by formation of disulfide bridges. Here we investigate another linking interaction that involves sulfhydryl groups of cysteines, namely intra- and intermolecular methylene-bridges between cysteine and lysine residues. A number of crystal structures possessing such a linkage were identified in the Protein Data Bank. Inspection of the electron density maps and re-refinement of the nominated structures unequivocally confirmed the presence of Lys-CH2 -Cys bonds in several cases.

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