Enhanced Prion Detection in Biological Samples by Magnetic Particle Extraction and Real-time Quaking-induced Conversion

Overview

Journal J Gen Virol

Specialty Microbiology

Date 2016 May 29

PMID 27233771

Citations 31

Authors

Nathaniel D Denkers

Davin M Henderson

Candace K Mathiason

Edward A Hoover

Affiliations

Soon will be listed here.

Abstract

Prions have been demonstrated in body fluids and excreta using bioassay, but at levels too low for detection by conventional direct-detection assays. More rapid and sensitive detection of prions in these clinically accessible specimens would be valuable for diagnosis and investigations of transmission, environmental impact, and interventions. In addition to very low concentrations of prions, in vitro amplification assays are challenged by the presence of inhibitors in these complex sources. Here, we leverage the prion attribute of avid metal binding with the versatile power of real-time quaking-induced conversion (RT-QuIC) to enhance and simplify detection of chronic wasting-disease prions in biological samples. Iron oxide particle binding and magnetic extraction combined with RT-QuIC permitted rapid analysis of the low concentrations of prions in saliva, urine, faeces, and cerebrospinal fluid. These methods are pertinent to ante-mortem detection, monitoring, and surveillance, and could conceivably be applicable to other protein-misfolding disorders.

Citing Articles

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Sandoval A, Nalls A, McNulty E, Denkers N, Trujillo D, Olmstead Z bioRxiv. 2025; .

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Evaluating the Diagnostic Efficacy of Using Pooled Samples for Chronic Wasting Disease Testing and Surveillance.

Hepker M, Zhang J, Anantharam V, Kanthasamy A, Yuan J, Zou W Pathogens. 2025; 13(12.

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Comparative study of immunoassays, a microelectromechanical systems-based biosensor, and RT-QuIC for the diagnosis of chronic wasting disease in white-tailed deer.

Kobashigawa E, Muhsin S, Abdullah A, Allen K, Sinnott E, Zhang M BMC Vet Res. 2024; 20(1):518.

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Temporal Characterization of Prion Shedding in Secreta of White-Tailed Deer in Longitudinal Study of Chronic Wasting Disease, United States.

Denkers N, McNulty E, Kraft C, Nalls A, Westrich J, Hoover E Emerg Infect Dis. 2024; 30(10):2118-2127.

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Prion Seeding Activity in Plant Tissues Detected by RT-QuIC.

Burgener K, Lichtenberg S, Walsh D, Inzalaco H, Lomax A, Pedersen J Pathogens. 2024; 13(6).

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