Bioactive Constituents of Zanthoxylum Rhetsa Bark and Its Cytotoxic Potential Against B16-F10 Melanoma Cancer and Normal Human Dermal Fibroblast (HDF) Cell Lines

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2016 May 28

PMID 27231889

Citations 8

Authors

Ramesh Kumar Santhanam

Syahida Ahmad

Faridah Abas

Intan Safinar Ismail

Yaya Rukayadi

Muhammad Tayyab Akhtar

Khozirah Shaari

Affiliations

Soon will be listed here.

Abstract

Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells.

Citing Articles

Assessing anti oxidant, antidiabetic potential and GCMS profiling of ethanolic root bark extract of Zanthoxylum rhetsa (Roxb.) DC: Supported by in vitro, in vivo and in silico molecular modeling.

Barman A, Mahadi S, Hossain M, Begum R, Acharyya R, Alam M PLoS One. 2024; 19(8):e0304521.

PMID: 39159188 PMC: 11332921. DOI: 10.1371/journal.pone.0304521.

Dietary Replacement of Soybean Meal with Seed Meal on Growth Performance, Blood Parameters, and Nutrient Utilization in Broiler Chickens.

Chen X, Li Y, Zheng A, Wang Z, Wei X, Li S Animals (Basel). 2024; 14(10).

PMID: 38791638 PMC: 11117249. DOI: 10.3390/ani14101420.

Recent Advances in the Antiproliferative and Proapoptotic Activity of Various Plant Extracts and Constituents against Murine Malignant Melanoma.

Dumitras D, Andrei S Molecules. 2022; 27(8).

PMID: 35458783 PMC: 9028509. DOI: 10.3390/molecules27082585.

Species: A Review of Traditional Uses, Phytochemistry and Pharmacology in Relation to Cancer, Infectious Diseases and Sickle Cell Anemia.

Okagu I, Ndefo J, Aham E, Udenigwe C Front Pharmacol. 2021; 12:713090.

PMID: 34603027 PMC: 8479109. DOI: 10.3389/fphar.2021.713090.

Simultaneous Qualitative and Quantitative Evaluation of the Coptidis Rhizoma and Euodiae Fructus Herbal Pair by Using UHPLC-ESI-QTOF-MS and UHPLC-DAD.

Li Y, Guo M, Li X, Yang X Molecules. 2020; 25(20).

PMID: 33081031 PMC: 7587604. DOI: 10.3390/molecules25204782.

References

Iwashita K, Kobori M, Yamaki K, Tsushida T . Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells. Biosci Biotechnol Biochem. 2000; 64(9):1813-20. DOI: 10.1271/bbb.64.1813. View

Ahsan M, Zaman T, Hasan C, Ito C, Islam S . Constituents and cytotoxicity of Zanthoxylum rhesta stem bark. Fitoterapia. 2000; 71(6):697-700. DOI: 10.1016/s0367-326x(00)00214-8. View

Albert A . FUNDAMENTAL ASPECTS OF SELECTIVE TOXICITY. Ann N Y Acad Sci. 1965; 123:5-18. DOI: 10.1111/j.1749-6632.1965.tb12241.x. View

Paik S, Koh K, Beak S, Paek S, Kim J . The essential oils from Zanthoxylum schinifolium pericarp induce apoptosis of HepG2 human hepatoma cells through increased production of reactive oxygen species. Biol Pharm Bull. 2005; 28(5):802-7. DOI: 10.1248/bpb.28.802. View

Kim J, Lim H, Lee D, Kim J, Kim D, Lee H . In vitro anti-inflammatory activity of lignans isolated from Magnolia fargesii. Bioorg Med Chem Lett. 2008; 19(3):937-40. DOI: 10.1016/j.bmcl.2008.11.103. View

Sung J, Park S, Seo D, Lee J, Hong S, Hong S . Antioxidative and skin-whitening effect of an aqueous extract of Salicornia herbacea. Biosci Biotechnol Biochem. 2009; 73(3):552-6. DOI: 10.1271/bbb.80601. View

Saleem M . Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett. 2009; 285(2):109-15. PMC: 2764818. DOI: 10.1016/j.canlet.2009.04.033. View

Yang C, Cheng M, Lee S, Yang C, Chang H, Chen I . Secondary metabolites and cytotoxic activities from the stem bark of Zanthoxylum nitidum. Chem Biodivers. 2009; 6(6):846-57. DOI: 10.1002/cbdv.200800107. View

Kim J, Jang Y, Kim B, Hwang K, Lee J . Induction of caspase-dependent apoptosis in melanoma cells by the synthetic compound (E)-1-(3,4-dihydroxyphenethyl)-3-styrylurea. BMB Rep. 2010; 42(12):806-11. DOI: 10.5483/bmbrep.2009.42.12.806. View

10.

Tantapakul C, Phakhodee W, Ritthiwigrom T, Yossathera K, Deachathai S, Laphookhieo S . Antibacterial compounds from Zanthoxylum rhetsa. Arch Pharm Res. 2012; 35(7):1139-42. DOI: 10.1007/s12272-012-0703-9. View

11.

Li W, Sun Y, Yan X, Yang S, Kim E, Kang H . Coumarins and lignans from Zanthoxylum schinifolium and their anticancer activities. J Agric Food Chem. 2013; 61(45):10730-40. DOI: 10.1021/jf403479c. View

12.

Mukhija M, Dhar K, Kalia A . Bioactive Lignans from Zanthoxylum alatum Roxb. stem bark with cytotoxic potential. J Ethnopharmacol. 2014; 152(1):106-12. DOI: 10.1016/j.jep.2013.12.039. View

13.

Kumar V, Kumar S, Singh B, Kumar N . Quantitative and structural analysis of amides and lignans in Zanthoxylum armatum by UPLC-DAD-ESI-QTOF-MS/MS. J Pharm Biomed Anal. 2014; 94:23-9. DOI: 10.1016/j.jpba.2014.01.028. View

14.

Ahsan M, Haque M, Hossain M, Islam S, Gray A, Hasan C . Cytotoxic dimeric quinolone-terpene alkaloids from the root bark of Zanthoxylum rhetsa. Phytochemistry. 2014; 103:8-12. DOI: 10.1016/j.phytochem.2014.03.008. View

15.

Zhang L, Ma L, Yan D, Zhang C, Gao D, Xiong Y . Dynamic monitoring of the cytotoxic effects of protoberberine alkaloids from Rhizoma Coptidis on HepG2 cells using the xCELLigence system. Chin J Nat Med. 2014; 12(6):428-35. DOI: 10.1016/S1875-5364(14)60067-4. View

16.

Chinembiri T, du Plessis L, Gerber M, Hamman J, Du Plessis J . Review of natural compounds for potential skin cancer treatment. Molecules. 2014; 19(8):11679-721. PMC: 6271439. DOI: 10.3390/molecules190811679. View

17.

Sandjo L, Kuete V, Tchangna R, Efferth T, Ngadjui B . Cytotoxic Benzophenanthridine and Furoquinoline Alkaloids from Zanthoxylum buesgenii (Rutaceae). Chem Cent J. 2014; 8(1):61. PMC: 4207896. DOI: 10.1186/s13065-014-0061-4. View

18.

Lan Y, Li H, Chen Y, Zhang Y, Liu N, Zhang Q . Essential oil from Zanthoxylum bungeanum Maxim. and its main components used as transdermal penetration enhancers: a comparative study. J Zhejiang Univ Sci B. 2014; 15(11):940-52. PMC: 4228507. DOI: 10.1631/jzus.B1400158. View