A Method to Rapidly Create Protein Aggregates in Living Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2016 May 28

PMID 27229621

Citations 17

Authors

Yusuke Miyazaki

Kota Mizumoto

Gautam Dey

Takamasa Kudo

John Perrino

Ling-Chun Chen

Tobias Meyer

Thomas J Wandless

Affiliations

Soon will be listed here.

Abstract

The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.

Citing Articles

Temporal control of acute protein aggregate turnover by UBE3C and NRF1-dependent proteasomal pathways.

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Aggregation and Oligomerization Characterization of ß-Lactoglobulin Protein Using a Solid-State Nanopore Sensor.

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Protein quality control and aggregation in the endoplasmic reticulum: From basic to bedside.

Chen G, Wei T, Ju F, Li H Front Cell Dev Biol. 2023; 11:1156152.

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A spider silk-derived solubility domain inhibits nuclear and cytosolic protein aggregation in human cells.

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