The Impact of Yeast Starter Cultures on the Microbial Communities and Volatile Compounds in Cocoa Fermentation and the Resulting Sensory Attributes of Chocolate

Overview

Journal J Food Sci Technol

Specialty Nutritional Sciences

Date 2016 May 11

PMID 27162390

Citations 23

Authors

Nadia Nara Batista

Cintia Lacerda Ramos

Disney Ribeiro Dias

Ana Carla Marques Pinheiro

Rosane Freitas Schwan

Affiliations

Soon will be listed here.

Abstract

Theobroma cacao seeds are the main raw material for chocolate production. During their fermentation, a succession of microorganisms are responsible for the physicochemical changes occurring in the pulp and inside the beans. The aim of this study was to investigate the effects of yeast inoculation (Saccharomyces cerevisiae UFLA CA11, Pichia kluivery CCMA0237, and Hanseniaspora uvarum CCMA0236) on the profile of the volatile compounds and microbial communities in cocoa fermentation. The resulting chocolate was also evaluated by temporal dominance of sensations (TDS) analyses. The dominant microorganisms during spontaneous fermentation were S. cerevisiae, H. uvarum, H. guilliermondii, Lactobacillus fermentum, Pediococcus sp., and Acetobacter pasteurianus. Similarly, S. cerevisiae, P. kluyveri, Candida sp., Pediococcus sp., and A. pasteurianus were the predominant microorganisms assessed by Denaturing Gradient Gel Electrophoresis (DGGE) in inoculated fermentation. Sixty-seven volatile compounds were detected and quantified by gas chromatography/mass spectrometry (GC/MS) at the end of fermentation and chocolates. The main group of volatile compound found after the inoculated and spontaneous fermentations was esters (41 and 39 %, respectively). In the chocolates, the main group was acids (73 and 44 % from the inoculated and spontaneous fermentations, respectively). The TDS analyses showed a dominance of bitter and cocoa attributes in both chocolates. However, in the inoculated chocolate, lingering fruity notes were more intense, while the chocolate produced by spontaneous fermentation was more astringent. Thus, the inoculation of yeast influenced the microbial profile, which likely affected the volatile compounds that affect sensory characteristics, resulting in chocolate with dominant bitter, cocoa, and fruity attributes.

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References

Ardhana M, Fleet G . The microbial ecology of cocoa bean fermentations in Indonesia. Int J Food Microbiol. 2003; 86(1-2):87-99. DOI: 10.1016/s0168-1605(03)00081-3. View

Schwan R, Wheals A . The microbiology of cocoa fermentation and its role in chocolate quality. Crit Rev Food Sci Nutr. 2004; 44(4):205-21. DOI: 10.1080/10408690490464104. View

Nielsen D, Teniola O, Ban-Koffi L, Owusu M, Andersson T, Holzapfel W . The microbiology of Ghanaian cocoa fermentations analysed using culture-dependent and culture-independent methods. Int J Food Microbiol. 2006; 114(2):168-86. DOI: 10.1016/j.ijfoodmicro.2006.09.010. View

Camu N, De Winter T, Verbrugghe K, Cleenwerck I, Vandamme P, Takrama J . Dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in Ghana. Appl Environ Microbiol. 2007; 73(6):1809-24. PMC: 1828797. DOI: 10.1128/AEM.02189-06. View

Camu N, Gonzalez A, De Winter T, Van Schoor A, De Bruyne K, Vandamme P . Influence of turning and environmental contamination on the dynamics of populations of lactic acid and acetic acid bacteria involved in spontaneous cocoa bean heap fermentation in Ghana. Appl Environ Microbiol. 2007; 74(1):86-98. PMC: 2223199. DOI: 10.1128/AEM.01512-07. View

Afoakwa E, Paterson A, Fowler M, Ryan A . Flavor formation and character in cocoa and chocolate: a critical review. Crit Rev Food Sci Nutr. 2008; 48(9):840-57. DOI: 10.1080/10408390701719272. View

Frauendorfer F, Schieberle P . Changes in key aroma compounds of Criollo cocoa beans during roasting. J Agric Food Chem. 2008; 56(21):10244-51. DOI: 10.1021/jf802098f. View

Ramos C, Gonzaga de Almeida E, de Melo Pereira G, Cardoso P, Dias E, Schwan R . Determination of dynamic characteristics of microbiota in a fermented beverage produced by Brazilian Amerindians using culture-dependent and culture-independent methods. Int J Food Microbiol. 2010; 140(2-3):225-31. DOI: 10.1016/j.ijfoodmicro.2010.03.029. View

Garcia-Armisen T, Papalexandratou Z, Hendryckx H, Camu N, Vrancken G, De Vuyst L . Diversity of the total bacterial community associated with Ghanaian and Brazilian cocoa bean fermentation samples as revealed by a 16 S rRNA gene clone library. Appl Microbiol Biotechnol. 2010; 87(6):2281-92. DOI: 10.1007/s00253-010-2698-9. View

10.

Aculey P, Snitkjaer P, Owusu M, Bassompiere M, Takrama J, Norgaard L . Ghanaian cocoa bean fermentation characterized by spectroscopic and chromatographic methods and chemometrics. J Food Sci. 2010; 75(6):S300-7. DOI: 10.1111/j.1750-3841.2010.01710.x. View

11.

Lefeber T, Janssens M, Camu N, De Vuyst L . Kinetic analysis of strains of lactic acid bacteria and acetic acid bacteria in cocoa pulp simulation media toward development of a starter culture for cocoa bean fermentation. Appl Environ Microbiol. 2010; 76(23):7708-16. PMC: 2988588. DOI: 10.1128/AEM.01206-10. View

12.

Lima L, Almeida M, Nout M, Zwietering M . Theobroma cacao L., "The food of the Gods": quality determinants of commercial cocoa beans, with particular reference to the impact of fermentation. Crit Rev Food Sci Nutr. 2011; 51(8):731-61. DOI: 10.1080/10408391003799913. View

13.

Lefeber T, Papalexandratou Z, Gobert W, Camu N, De Vuyst L . On-farm implementation of a starter culture for improved cocoa bean fermentation and its influence on the flavour of chocolates produced thereof. Food Microbiol. 2012; 30(2):379-92. DOI: 10.1016/j.fm.2011.12.021. View

14.

de Melo Pereira G, da Cruz Pedrozo Miguel M, Ramos C, Schwan R . Microbiological and physicochemical characterization of small-scale cocoa fermentations and screening of yeast and bacterial strains to develop a defined starter culture. Appl Environ Microbiol. 2012; 78(15):5395-405. PMC: 3416416. DOI: 10.1128/AEM.01144-12. View

15.

Crafack M, Mikkelsen M, Saerens S, Knudsen M, Blennow A, Lowor S . Influencing cocoa flavour using Pichia kluyveri and Kluyveromyces marxianus in a defined mixed starter culture for cocoa fermentation. Int J Food Microbiol. 2013; 167(1):103-16. DOI: 10.1016/j.ijfoodmicro.2013.06.024. View

16.

Moens F, Lefeber T, De Vuyst L . Oxidation of metabolites highlights the microbial interactions and role of Acetobacter pasteurianus during cocoa bean fermentation. Appl Environ Microbiol. 2014; 80(6):1848-57. PMC: 3957632. DOI: 10.1128/AEM.03344-13. View

17.

Owusu M, Agerlin Petersen M, Heimdal H . Relationship of sensory and instrumental aroma measurements of dark chocolate as influenced by fermentation method, roasting and conching conditions. J Food Sci Technol. 2014; 50(5):909-17. PMC: 3722411. DOI: 10.1007/s13197-011-0420-2. View

18.

Ho V, Zhao J, Fleet G . Yeasts are essential for cocoa bean fermentation. Int J Food Microbiol. 2014; 174:72-87. DOI: 10.1016/j.ijfoodmicro.2013.12.014. View

19.

Belscak-Cvitanovic A, Komes D, Dujmovic M, Karlovic S, Biskic M, Brncic M . Physical, bioactive and sensory quality parameters of reduced sugar chocolates formulated with natural sweeteners as sucrose alternatives. Food Chem. 2014; 167:61-70. DOI: 10.1016/j.foodchem.2014.06.064. View

20.

Nolvachai Y, Kulsing C, Boysen R, Matyska M, Pesek J, Marriott P . Comparison of the performance of different silica hydride particles for the solid-phase extraction of non-volatile analytes from dark chocolate with analysis by gas chromatography-quadrupole mass spectrometry. Food Chem. 2014; 174:434-9. DOI: 10.1016/j.foodchem.2014.10.083. View